Lidstrom:Digestion with Restriction Enzymes

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(New page: Back to Protocols *Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends: 10 uL DNA 5uL buffer (check h...)
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*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
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10 uL DNA
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**10 uL DNA
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5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
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**5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
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0.5 uL BSA (if required. See above)
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**0.5 uL BSA (if required. See above)
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1uL enzyme 1
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**1uL enzyme 1
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water to 50 uL (32.5 uL, add first)
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**water to 50 uL (32.5 uL, add first)
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If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
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**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.

Revision as of 16:02, 6 March 2012

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