Lidstrom:Competent Cell Preparation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Back to Protocols ==Chemically Competent E. Coli== Notes: *You need fresh cells. **Often people inoculate a few mL of the culture for overnight growth, then use 20...) |
(No difference)
|
Revision as of 15:33, 10 April 2012
Back to Protocols
Chemically Competent E. Coli
Notes:
- You need fresh cells.
- Often people inoculate a few mL of the culture for overnight growth, then use 200 uL to inoculate the ~50 mL of culture they will make competent.
- Some people in our lab believe you want to start with a fresh plate and don't even use one that is a few days old. Other people (Mila) are mostly concerned about the OD being right at the time of harvest.
ElectroCompetent E. Coli
- This is not done frequently in our lab.
