Lidstrom:Colony PCR

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(New page: ==General:== *The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC. *The annealing temperature is up to y...)
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==General:==
==General:==
*The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit.  I have seen 68oC & 72oC.  
*The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit.  I have seen 68oC & 72oC.  

Revision as of 18:12, 2 February 2012

Back to Protocols

General:

  • The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC.
  • The annealing temperature is up to you. For VF2 & VR 54-56oC works well.
  • The primer concentration varies in different people's recipes.
  • Janet is using NEB's OneTaq quick-load (not hot start) & their recommended protocol
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