Lidstrom:Choosing a protein concentration quantification method

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Major methods available

• Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
  • fastest/easiest but may have most bias (??)
  • Ceci/Amanda/Frances chose BCA instead.
• Pierce brand BCA assay

Compatibility with your lysis solution

protein assay compatibility table from Pierce

Resources

• Thermo Scientific: Overview of Protein Assays Methods
• Thermo Scientific: Protein Assay Selection Guide
• Thermo Scientific: Protein Assay Technical Handbook