Lidstrom:Choosing a protein concentration quantification method

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Compatibility with your lysis solution)
Line 1: Line 1:
Back to [[Lidstrom:Protocols|Protocols]]
Back to [[Lidstrom:Protocols|Protocols]]
 +
 +
=== Major methods available ===
 +
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
 +
** fastest/easiest but may have most bias (??)
 +
** Ceci/Amanda/Frances chose BCA instead.
 +
* Pierce brand BCA assay
=== Compatibility with your lysis solution ===
=== Compatibility with your lysis solution ===
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]

Revision as of 08:38, 30 October 2013

Back to Protocols

Major methods available

  • Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
    • fastest/easiest but may have most bias (??)
    • Ceci/Amanda/Frances chose BCA instead.
  • Pierce brand BCA assay

Compatibility with your lysis solution

protein assay compatibility table from Pierce
protein assay compatibility table from Pierce
Personal tools