Lidstrom:Choosing a protein concentration quantification method

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* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods]
* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods]
* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide]
* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide]
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* [http://www.piercenet.com/page/protein-assay-handbook-1602063 Thermo Scientific: Protein Assay Technical Handbook]

Revision as of 09:24, 30 October 2013

Back to Protocols

Major methods available

  • Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
    • fastest/easiest but may have most bias (??)
    • Ceci/Amanda/Frances chose BCA instead.
  • Pierce brand BCA assay

Compatibility with your lysis solution

protein assay compatibility table from Pierce
protein assay compatibility table from Pierce

Resources

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