Lidstrom:Choosing a protein concentration quantification method: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Back to [[Lidstrom:Protocols|Protocols]] | Back to [[Lidstrom:Protocols|Protocols]] | ||
== Major methods available == | |||
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances) | * Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances) | ||
** fastest/easiest but may have most bias (??) | ** fastest/easiest but may have most bias (??) | ||
| Line 7: | Line 7: | ||
* Pierce brand BCA assay | * Pierce brand BCA assay | ||
== Compatibility with your lysis solution == | |||
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]] | [[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]] | ||
== Resources == | |||
* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods] | |||
* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide] | |||
Revision as of 07:24, 30 October 2013
Back to Protocols
Major methods available
- Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
- fastest/easiest but may have most bias (??)
- Ceci/Amanda/Frances chose BCA instead.
- Pierce brand BCA assay
Compatibility with your lysis solution
