Lidstrom:Choosing a protein concentration quantification method

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Back to [[Lidstrom:Protocols|Protocols]]
Back to [[Lidstrom:Protocols|Protocols]]
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=== Major methods available ===
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== Major methods available ==
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
** fastest/easiest but may have most bias (??)  
** fastest/easiest but may have most bias (??)  
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* Pierce brand BCA assay
* Pierce brand BCA assay
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=== Compatibility with your lysis solution ===
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== Compatibility with your lysis solution ==
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]
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== Resources ==
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* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods]
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* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide]

Revision as of 09:24, 30 October 2013

Back to Protocols

Major methods available

  • Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
    • fastest/easiest but may have most bias (??)
    • Ceci/Amanda/Frances chose BCA instead.
  • Pierce brand BCA assay

Compatibility with your lysis solution

protein assay compatibility table from Pierce
protein assay compatibility table from Pierce

Resources

Personal tools