Lidstrom:Back Door:Useful Links: Difference between revisions
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Back to [[Lidstrom:Back Door|Back Door]] | Back to [[Lidstrom:Back Door|Back Door]] | ||
To [[Lidstrom:Protocols|Protocols]] | |||
==General== | ==General== | ||
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*[http://www.gene-quantification.de/Qiagen-BenchGuide.pdf Qiagen Bench Guide] | *[http://www.gene-quantification.de/Qiagen-BenchGuide.pdf Qiagen Bench Guide] | ||
** cell culture & DNA | ** cell culture & DNA | ||
* Plasmid guide: Plasmids 101 | |||
** Download from: http://info.addgene.org/download-addgenes-ebook-plasmids-101-1st-edition | |||
==Microbiology== | ==Microbiology== | ||
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===DNA/RNA Tools=== | ===DNA/RNA Tools=== | ||
=== Cloning guides ==== | |||
* [https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Guide_1113.pdf NEB Molecular Cloning Technical Guide]: overview and comparison of most types of cloning. | |||
==== plasmid annotations ==== | ==== plasmid annotations ==== | ||
*[http://biologylabs.utah.edu/jorgensen/wayned/ape/ APE] free plasmid editor | *[http://biologylabs.utah.edu/jorgensen/wayned/ape/ APE] free plasmid editor | ||
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===Protein Tools=== | ===Protein Tools=== | ||
==== Size Estimation==== | |||
** [http://www.bioinformatics.org/sms/prot_mw.html Protein MW Calculator] | ** [http://www.bioinformatics.org/sms/prot_mw.html Protein MW Calculator] | ||
** [http://molbiol.ru/eng/scripts/01_04.html Protein Mass to Mol Converter] | ** [http://molbiol.ru/eng/scripts/01_04.html Protein Mass to Mol Converter] | ||
==== Visualization ==== | |||
** | [http://www.pymol.org/ Pymol] | ||
** | * Tutorials: | ||
** | ** [http://www.pymolwiki.org/index.php/Practical_Pymol_for_Beginners pymol for beginners] | ||
** | ** [http://bioquest.org/nimbios2010/wp-content/blogs.dir/files/2010/07/pymol_tutorial3.pdf bioquest.org]. | ||
**[http://www.cgl.ucsf.edu/chimera/download.html Chimera] | ** [http://www.doe-mbi.ucla.edu/CHEM125/pymol_tutorial_060418.pdf UCLA]: concise less basic review | ||
** [http://bioquest.org/nimbios2010/wp-content/blogs.dir/files/2010/07/pymol_tutorial3.pdf Madison Tutorial] | |||
* Scripting | |||
** [http://www.bio.ph.ic.ac.uk/~scurry/pdfs/PyMOL_tutorial.pdf Quickie PyMol Tutorial]: great script syntax examples | |||
* Cheat sheets: | |||
** intermediate level: [http://www.medschool.lsuhsc.edu/biochemistry/docs/PyMOL-QuickRef-0905.pdf AMAZING Cheat Sheet] | |||
** [http://www.doe-mbi.ucla.edu/CHEM125/PymolRef.pdf UCLA], 2 pages of text | |||
* tips/examples: | |||
** [http://www-cryst.bioc.cam.ac.uk/members/zbyszek/figures_pymol figure tips] | |||
*[http://www.cgl.ucsf.edu/chimera/download.html Chimera] | |||
==== Structure Prediction ==== | |||
*[http://swissmodel.expasy.org/ Swiss Model] | |||
*[http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index Phyre] | |||
==== Alignment ==== | |||
*[http://fatcat.burnham.org/ FATCAT] | |||
=== Enzymes === | === Enzymes === | ||
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R | R | ||
* tutorials: | * tutorials: | ||
** [http://swirlstats.com/ Swirl]: swirl teaches you R programming and data science interactively, at your own pace, and right in the R console | |||
*** [https://github.com/swirldev/swirl_courses#swirl-courses List of courses within Swirl] | |||
** [https://www.datacamp.com/ R tutorial] | ** [https://www.datacamp.com/ R tutorial] | ||
** From [https://github.com/raphg/Biostat-578 Biostat 578] | ** From [https://github.com/raphg/Biostat-578 Biostat 578] | ||
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** [http://adv-r.had.co.nz/ Advanced R] by the author of ggplot, etc. (Hadley Wickham) | ** [http://adv-r.had.co.nz/ Advanced R] by the author of ggplot, etc. (Hadley Wickham) | ||
* [http://r-project.org Bioconductor] | * [http://r-project.org Bioconductor] | ||
* [http://www.cookbook-r.com/Graphs/] amazing reference for making ggplot graphs! | |||
Regular expressions: | Regular expressions: | ||
*[http://pythex.org/ PyThex]. A quick way to test your Python regular expressions. | |||
*[http://rubular.com/ Rubular]. Website to test regular expressions in Ruby, but works for grep in R. It is possible it works a little differently in Ruby and grep. | *[http://rubular.com/ Rubular]. Website to test regular expressions in Ruby, but works for grep in R. It is possible it works a little differently in Ruby and grep. | ||
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** [http://www.wolfram.com/learningcenter/tutorialcollection/MathematicsAndAlgorithms/MathematicsAndAlgorithms.pdf Excellent equation solving tutorial] | ** [http://www.wolfram.com/learningcenter/tutorialcollection/MathematicsAndAlgorithms/MathematicsAndAlgorithms.pdf Excellent equation solving tutorial] | ||
* [http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20chemical%20mutagenesis.pdf chemical mutagenesis summary] of popular mutagens | * [http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20chemical%20mutagenesis.pdf chemical mutagenesis summary] of popular mutagens | ||
* Site directed mutagenesis: | |||
** QuikChange: | |||
*** The Agilent commercial kits for single and multiple mutations work great. [[Richard_Lab:Site_Directed_Mutagenesis|This protocol]] should deliver the same results with a bit lower efficiency and slower Dpn1 step but is almost free. | |||
** Free [https://www.genomics.agilent.com/primerDesignProgram.jsp Agilent QuikChange primer design tool] (Agilent account required) | |||
** QuikChange manuals: | |||
*** [http://www.chem.agilent.com/library/usermanuals/Public/210514.pdf Lightning, Multi Kit] | |||
**** Catalog # 210514 (10 reactions) and #210516 (30 reactions) | |||
**** Academic version of kit (cheaper): Item # 210515 ($321.30, 10 rxns), Item # 210513 ($853.20, 30 rxns) | |||
*** [http://www.chem.agilent.com/library/usermanuals/Public/210518.pdf Lightning, Single mutation at a time] | |||
***** Catalog # 210518 (10 reactions) and #210519 (30 reactions). No academic price appears to be available ([[User:Janet B. Matsen|JM]] 12 June 2014) | |||
* [http://eu.idtdna.com/pages/docs/default-source/user-guides-and-protocols/mutagenesis-application-guide.pdf?sfvrsn=9 IDT Mutagenesis Application Guide] for mutating genes | |||
== Organic Chemistry == | |||
* Free downloadable book with focus on biology: download [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis here] | |||
== Misc == | == Misc == | ||
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==Presentations/Posters== | ==Presentations/Posters== | ||
*[http://www.vischeck.com/vischeck/vischeckImage.php Website] to see how colorblind people will see your slides | *[http://www.vischeck.com/vischeck/vischeckImage.php Website] to see how colorblind people will see your slides | ||
*[https://www.washington.edu/externalaffairs/uwmarketing/RBI/docs/UniversityArtPack.zip University Art Pack Download]: a collection of University of Washington logos | *[https://www.washington.edu/externalaffairs/uwmarketing/RBI/docs/UniversityArtPack.zip University Art Pack Download]: a collection of University of Washington logos | ||
* [https://inkscape.org/en/ Inkscape] (free version of Adobe Illustrator) | |||
==University of Washington== | ==University of Washington== | ||
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** [http://arstechnica.com/business/2012/05/hands-on-testing-the-gimp-28-and-its-new-single-window-interface/ website] about the interface | ** [http://arstechnica.com/business/2012/05/hands-on-testing-the-gimp-28-and-its-new-single-window-interface/ website] about the interface | ||
** http://www.gimp.org/release-notes/gimp-2.8.html - shows the location plus download link | ** http://www.gimp.org/release-notes/gimp-2.8.html - shows the location plus download link | ||
* colors | |||
** [http://colorbrewer2.org/ ColorBrewer] - provides nice color palettes & helps you create hex colors | |||
** [http://www.perbang.dk/rgbgradient/ Hex gradient creator] | |||
* drawing chemical structures: (ask Amanda about either of these) | * drawing chemical structures: (ask Amanda about either of these) | ||
** [http://www.acdlabs.com/resources/freeware/chemsketch/ chemsketch] (free) | ** [http://www.acdlabs.com/resources/freeware/chemsketch/ chemsketch] (free) | ||
** [http://www.cambridgesoft.com/Ensemble_for_Chemistry/ChemDraw/ chemdraw] (seems to cost $) | ** [http://www.cambridgesoft.com/Ensemble_for_Chemistry/ChemDraw/ chemdraw] (seems to cost $) | ||
== Statistics == | |||
=== Multiple linear regression === | |||
* [http://math.arizona.edu/~hzhang/waeso/multlr.pdf basics, from an R perspective]. | |||
* Relative importance of variables: | |||
** [http://www.unt.edu/rss/class/mike/5710/Multiple%20Regression.pdf good lecture slides] | |||
=== Statistical testing === | |||
*[http://www.statisticsdonewrong.com/introduction.html Statistics done wrong] Good explanation of p-value | |||
==Industry== | |||
* [http://www.biofuelsdigest.com/bdigest/2014/05/20/no-shortcuts-to-the-top-a-digest-special-report-on-scale-up-in-industrial-biotechnology/ scale up slides] from major players | |||
==Misc== | ==Misc== |
Revision as of 06:41, 5 May 2015
Back to Back Door
To Protocols
General
- general biology/biotech basics from Integrated DNA Technologies (IDT)
- Molecular Biology Background:
- Mitochondrial DNA, Mendelian Genetics, Molecular Facts and Figures, Protein Synthesis, microRNAs, DNA Replication, A Brief History of DNA, What is an Oligo
- Molecular Biology Techniques:
- The Polymerase Chain Reaction, Gel Electrophoresis , Restriction Endonucleases , DNA Sequencing , Antisense Technologies , A Basic PCR Protocol
- Molecular Biology Background:
- Qiagen Bench Guide
- cell culture & DNA
- Plasmid guide: Plasmids 101
Microbiology
E. coli
- Working with E. coli
- coli Genome Stock Center
- Ecocyc
- EcoSal !!subscription required!! (not available at UW)
- origins of replication
- RegulonDB A curated database of E. coli regulation.
Molecular Biology
DNA/RNA Tools
Cloning guides =
- NEB Molecular Cloning Technical Guide: overview and comparison of most types of cloning.
plasmid annotations
- APE free plasmid editor
- Serial Cloner free equivalent to Vector NTI
- PlasMapper
- The PlasMapper server automatically generates and annotates plasmid maps using only the plasmid DNA sequence as input. Plasmid figures may be rendered in PNG, JPG, SVG or SVGZ format.
codon optimization
- IDT's web tool to optimize for a given organism
- Doesn't provide any info about the strategy used for codon optimization. (Boo!) However, IDT is a leading company for DNA technology, so whatever they use is likely fine.
- OPTIMIZER
- Original paper
- Requires more selections, so is less simple than the IDT one.
- Genscript Rare Codon Analysis
codon frequency tables for E. coli
- from HÉNAUT and DANCHIN:Analysis and Predictions from Escherichia coli sequences
- Escherichia coli and Salmonella, Vol. 2, Ch. 114:2047-2066, 1996, Neidhardt FC ed., ASM press, Washington, D.C.
- Class I contains genes involved in most metabolic processes. Class II genes correspond to genes highly and continuously expressed during exponential growth.
- Table modified from Maloy, S., V. Stewart, and R. Taylor. 1996. Genetic analysis of pathogenic bacteria. Cold Spring Harbor Laboratory Press, NY.
other
- Agarose gel electrophoresis basics
- Genewiz DNA sequencing
- IDT DNA oligos
- EBI Sequence Alignment
- BLAST! Sequence Alignment
- The most commonly used nucleotide/protein alignment tool.
- start here (animated web intro)
- Practical intro: CHS protocols
- protein alignments: "The line between the two sequences indicates the similarities between the sequences. If the query and the subject have the same amino acid at a given location, the residue itself is shown. Conservative substitutions, as judged by the substitution matrix, are indicated with +."
- RBS strength calculator/engineering tool
- Another: link
- Accuracy stated by FAQ: "on average, the predictions of the RBS Calculator are accurate to within a factor of 2.3, equivalent to an error of 1.82 kcal/mol in the thermodynamic model. There is a 47% chance that a synthetic ribosome binding site will be accurate to within 2-fold of its predicted translation initiation rate." So you can estimate it to have accuracy within an order of magnitude. Pretty sensitive to the amount of upstream & downstream sequence info you provide. Check your forward engineering designs with more bp included in the reverse engineering mode.
- Finnzyme Tm calculator: use if you use Phusion DNA polymerase
- Finnzyme multiple primer analyzer looks for primer dimers (check before ordering primers)
- Replicons and Compatibility
- Nanodrop Info
- NuPack Nucleic acid secondary structure predictor
BioBricks
Protein Tools
Size Estimation
Visualization
- Tutorials:
- pymol for beginners
- bioquest.org.
- UCLA: concise less basic review
- Madison Tutorial
- Scripting
- Quickie PyMol Tutorial: great script syntax examples
- Cheat sheets:
- intermediate level: AMAZING Cheat Sheet
- UCLA, 2 pages of text
- tips/examples:
Structure Prediction
Alignment
Enzymes
Synthetic Biology
Mass Spec
HPLC
- Shimadzu Virtual Advisor.
- Ask Nicole, Amanda, or Janet for the lab log-in.
Programming/Scripting
R
- tutorials:
- Swirl: swirl teaches you R programming and data science interactively, at your own pace, and right in the R console
- R tutorial
- From Biostat 578
- Introductory Statistics (R) by Peter Dalgaard
- R tutorial
- references:
- cheat sheet
- reference card
- Advanced R by the author of ggplot, etc. (Hadley Wickham)
- Bioconductor
- [1] amazing reference for making ggplot graphs!
Regular expressions:
- PyThex. A quick way to test your Python regular expressions.
- Rubular. Website to test regular expressions in Ruby, but works for grep in R. It is possible it works a little differently in Ruby and grep.
GitHub
Protein Engineering
- Random mutagenesis methods summarized succinctly.
- chemical mutagenesis summary of popular mutagens
- Site directed mutagenesis:
- QuikChange:
- The Agilent commercial kits for single and multiple mutations work great. This protocol should deliver the same results with a bit lower efficiency and slower Dpn1 step but is almost free.
- Free Agilent QuikChange primer design tool (Agilent account required)
- QuikChange manuals:
- Lightning, Multi Kit
- Catalog # 210514 (10 reactions) and #210516 (30 reactions)
- Academic version of kit (cheaper): Item # 210515 ($321.30, 10 rxns), Item # 210513 ($853.20, 30 rxns)
- Lightning, Single mutation at a time
- Catalog # 210518 (10 reactions) and #210519 (30 reactions). No academic price appears to be available (JM 12 June 2014)
- Lightning, Multi Kit
- QuikChange:
- IDT Mutagenesis Application Guide for mutating genes
Organic Chemistry
- Free downloadable book with focus on biology: download here
Misc
- Mathematica (not free)
Presentations/Posters
- Website to see how colorblind people will see your slides
- University Art Pack Download: a collection of University of Washington logos
- Inkscape (free version of Adobe Illustrator)
University of Washington
UW Resources
Safety/Lab Technique
- Sample video: sterile technique
- biohazard waste stream flow chart UW
Graphics
- inkscape - SVG (vector) drawings, free. Loved by Janet & Amanda
- Inkscape: Guide to a Vector Drawing use examples
- GIMP - pixels (like photoshp), free
- website about the interface
- http://www.gimp.org/release-notes/gimp-2.8.html - shows the location plus download link
- colors
- ColorBrewer - provides nice color palettes & helps you create hex colors
- Hex gradient creator
- drawing chemical structures: (ask Amanda about either of these)
- chemsketch (free)
- chemdraw (seems to cost $)
Statistics
Multiple linear regression
- basics, from an R perspective.
- Relative importance of variables:
Statistical testing
- Statistics done wrong Good explanation of p-value
Industry
- scale up slides from major players
Misc
- Mendeley : reference/paper manager that's awesome
- Janet's workflow: (1) discover papers via google scholar saved search, (2) save them to Mendeley in the appropriate nested folder regardless of whether you intend to read it now or "some day when I'm thinking about that topic" (3) read in Mendeley, which makes a green dot (indicating unread) go away.
- You can highlight and keep notes on top of your PDFs enabling quicker review of topics.
- Imagine being able to query all of the papers you have ever read for a topic of interest! You can also see whether you have ever read a paper or not, potentially saving yourself time.
- I also like the idea that I will never lose track of a paper I want to tell someone about. It isn't uncommon for someone to tell me about a paper but be unable to find it and send it to me. This should never happen for a dedicated Mendeley user.
- Endy:Victor3_plate_reader/filters Filters for Fluorescence
- Equilibrator
- BioNumbers: a collection of useful bionumbers
- emolecules Chemical Lookup