Lerou:Research

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==Research Goals==
==Research Goals==
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Our research goals are to advance the fundamental understanding of pluripotent stem cell biology and to explore how failure of genomic integrity impacts human development. We are developing novel live-cell and fixed-cell imaging techniques to study mitotic progression and genomic stability in human pluripotent stem cells. These techniques will be coupled with single cell analysis and computational biology approaches to improve our understanding of how cell cycle regulation and pluripotency are coupled.
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Our research goals are to advance the fundamental understanding of pluripotent stem cell biology in order to unlock their therapeutic and research potential. To achieve this goal we will plan to apply novel cell imaging techniques and genomic approaches to explore differentiation dynamics in human pluripotent stem cells. These techniques are coupled with single cell analysis and computational biology approaches to improve our understanding of complex characteristics associated with human pluripotency.
==Research Interests==
==Research Interests==

Revision as of 15:26, 14 March 2014

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Embryonic Stem Cell Colony
Embryonic Stem Cell Colony

Research Goals

Our research goals are to advance the fundamental understanding of pluripotent stem cell biology in order to unlock their therapeutic and research potential. To achieve this goal we will plan to apply novel cell imaging techniques and genomic approaches to explore differentiation dynamics in human pluripotent stem cells. These techniques are coupled with single cell analysis and computational biology approaches to improve our understanding of complex characteristics associated with human pluripotency.

Research Interests

  • Human pluripotent stem cells: embryonic stem (ES) cells and induced pluripotency stem (iPS) cells
  • Genomic stability regulation in human pluripotent stem cells
  • Cell cycle regulation of human pluripotent stem cells
  • p53 dynamics of human pluripotent stem cells

Outcomes/Applications

Embryonic Stem Cells, DNA labeled with GFP
Embryonic Stem Cells, DNA labeled with GFP

The most common and clinically problematic form of genomic instability is aneuploidy, a hallmark of cancer and a cause of infertility and congenital developmental defects. The causes of aneuploidy remain largely unknown, yet the impact on development is profound. Aneuploidy affects 35% of clinically recognized spontaneous abortions, 4% of stillborns and 3 in 1000 live borns. As neonatologists, we witness firsthand the consequences of aneuploidy on development when we care for couples that have struggled with infertility and whose infants are afflicted by genetic syndromes. The long term goal of our research will be to use human pluripotent stem cell research to improve our understanding of early human development and how genomic instability disrupts normal development and contributes to birth defects.

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