Lauren M. Magee Week 2
Word Definitions
- permease-encoding
- biosynthetic
- assimilation
- extracellular
- intracellular
- flux
- biomass
- parameters
- cultured
- transferase
Paper Outline
Introduction
Ammonia is a preferred nitrogen source for Saccharomyces cerevisiae. Nitrogen metabolism is regulated at both the level of gene expression and the level of enzyme activity. Research has pointed out the influence of the concentration of ammonia and the rate of ammonia assimilation. Because of this interaction, the researchers hypothesized that the ammonia flux may have a larger impact than the concentration of ammonia in nitrogen metabolism. To study this, the researchers observed cultures having the same levels of flux but having feeds with different ammonia concentrations.
Material & Methods
S. cerevisiae was used to observe the effects of differing ammonia concentrations gene expression and enzyme activities. S. cerevisiae was continuously cultures and then feed ammonia in concentrations ranging from 29 to 118 mM along with a fixed glucose concentration and dilution rate.
Results
Discussion
References
Caveat Emptor
Lauren M. Magee
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