Lauren M. Magee Week 10

Group 1: Alyssa, Karina, Will, Lauren

• Tai, S. L., Daran-Lapujade, P., Walsh, M. C., Pronk, J. T., & Daran, J. M. (2007). Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis. Molecular biology of the cell, 18(12), 5100-5112. doi: 10.1091/mbc.E07-02-0131
• Link to PDF version of article

Biological Terms

1. Fluxes: a flow or flowing of a liquid
   • Source: http://www.thefreedictionary.com/fluxes
2. Biogenesis: the principle that living organisms develop only from other living organisms and not from nonliving matter.
   • Source: http://www.thefreedictionary.com/biogenesis
3. Trehalose: a sweet-tasting, crystalline disaccharide, C12H22O11, found in trehala, in the hemolymph of numerous insects, and in many fungi.
   • Source: http://www.thefreedictionary.com/trehalose
4. Suboptimal: being below an optimal level or standard.
   • Source: http://www.thefreedictionary.com/suboptimal
5. Chemostat: an apparatus for growing bacterial cultures at a constant rate by controlling the supply of nutrient medium.
   • Source: http://www.thefreedictionary.com/chemostat
6. Desaturase: an enzyme that removes two hydrogen atoms from a fatty acid, creating a carbon/carbon double bond.
   • Source: http://encyclopedia.thefreedictionary.com/desaturase
7. Prototrophic:
   • Citation
8. Cryostat:a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat.
   • Source: http://encyclopedia.thefreedictionary.com/cryostat
9. Immunoprecipitation: the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein.
   • Source: http://encyclopedia.thefreedictionary.com/Immunoprecipitation
10. Catabolism: The metabolic breakdown of complex molecules into simpler ones, often resulting in a release of energy.
    • Source: http://www.thefreedictionary.com/Catabolism

Article Outline

1. What is the main result presented in this paper?
2. What is the importance or significance of this work?
3. Briefly describe their methods, including the following information. A flow chart may be helpful here.
4. How did they treat the cells (what experiment were they doing?)
5. What strain(s) of yeast did they use? Was the strain haploid or diploid?
6. What media did they grow them in? Under what conditions and temperatures?
7. What controls did they use?
8. How many replicates did they perform per condition?
9. What mathematical/statistical method did they use to analyze the data?
10. What transcription factors did they talk about?
11. Briefly state the result shown in each of the figures and tables.