Laub:PYE: Difference between revisions
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==Instructions== | ==Instructions== | ||
Add ingredients and autoclave (250°F, 22psi, 30-60min depending on volume). | *Add ingredients and autoclave (250°F, 22psi, 30-60min depending on volume). | ||
*For plates, usually add 1.5% (w/v) BactoAgar as well. | |||
==Notes== | ==Notes== | ||
*If PYE is to be aliquoted, first prepare 1L as described, then aliquot/pour into 100mL bottles. Finally, reautoclave individual bottles | *If PYE is to be aliquoted, first prepare 1L as described, then aliquot/pour into 100mL bottles. Finally, reautoclave individual bottles | ||
Revision as of 23:28, 21 January 2007
Rich Media for Caulobacter crescentus
Ingredients
- 2.0g bactopeptone
- 1.0g yeast extract
- 0.3g MgSO4
- From Sigma
- 10mL 50 mM CaCl2
- From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks).
- 1L H2O (milliQ or distilled)
Instructions
- Add ingredients and autoclave (250°F, 22psi, 30-60min depending on volume).
- For plates, usually add 1.5% (w/v) BactoAgar as well.
Notes
- If PYE is to be aliquoted, first prepare 1L as described, then aliquot/pour into 100mL bottles. Finally, reautoclave individual bottles
