Lactobacillus transformation (Speer 2012)
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==Procedure== | ==Procedure== | ||
| - | + | #Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking). | |
| + | #Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine. | ||
| + | ##Shake to dissolve. | ||
| + | #Add 1ml overnight culture to each flask (1:50 dlution) | ||
| + | #Culture for ~3 hours. | ||
==Notes== | ==Notes== | ||
Revision as of 15:40, 14 September 2011
Back to Protocols
Back to Electro-transformation of Lactobacillus spp.
Contents |
Overview
This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.
Materials
- MRS media
- Glycine
- Milipore water
- 50mM EDTA solution
- 0.5M Sucrose, 10% glycerol solution
Procedure
- Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking).
- Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine.
- Shake to dissolve.
- Add 1ml overnight culture to each flask (1:50 dlution)
- Culture for ~3 hours.
Notes
All questions, input and feedback are welcome!
References
There will be one here soon.
Contact
- mas853@psu.edu
or instead, discuss this protocol. -->
