Lactobacillus transformation (Speer 2012)

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(Materials)
(Procedure)
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==Procedure==
==Procedure==
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#Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking).
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#Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine.
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##Shake to dissolve.
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#Add 1ml overnight culture to each flask (1:50 dlution)
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#Culture for ~3 hours.
==Notes==
==Notes==

Revision as of 15:40, 14 September 2011

Back to Protocols
Back to Electro-transformation of Lactobacillus spp.

Contents

Overview

This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.

Materials

  • MRS media
  • Glycine
  • Milipore water
  • 50mM EDTA solution
  • 0.5M Sucrose, 10% glycerol solution

Procedure

  1. Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking).
  2. Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine.
    1. Shake to dissolve.
  3. Add 1ml overnight culture to each flask (1:50 dlution)
  4. Culture for ~3 hours.

Notes

All questions, input and feedback are welcome!

References

There will be one here soon.


Contact

  • mas853@psu.edu

or instead, discuss this protocol. -->

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