Lactobacillus transformation (Speer 2012)
From OpenWetWare
(Difference between revisions)
(→Materials) |
|||
| Line 8: | Line 8: | ||
*Glycine | *Glycine | ||
*Milipore water | *Milipore water | ||
| - | + | *50mM EDTA solution | |
| + | *0.5M Sucrose, 10% glycerol solution | ||
==Procedure== | ==Procedure== | ||
Revision as of 15:21, 14 September 2011
Back to Protocols
Back to Electro-transformation of Lactobacillus spp.
Contents |
Overview
This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.
Materials
- MRS media
- Glycine
- Milipore water
- 50mM EDTA solution
- 0.5M Sucrose, 10% glycerol solution
Procedure
Notes
All questions, input and feedback are welcome!
References
There will be one here soon.
Contact
- mas853@psu.edu
or instead, discuss this protocol. -->
