Lactobacillus transformation (Speer 2012)

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Overview

This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.

Materials

• MRS media
• Glycine
• Milipore water
• 50mM EDTA solution
• 0.5M Sucrose, 10% glycerol solution

Procedure

1. Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking).
2. Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine.
   1. Shake to dissolve.
3. Add 1ml overnight culture to each flask (1:50 dlution)
4. Culture for ~3 hours.

Notes

All questions, input and feedback are welcome!

References

There will be one here soon.

Contact

• mas853@psu.edu

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