Lactobacillus planarum miniprep - Revision history

Michael A. Speer: /* Procedure */

Michael A. Speer — Fri, 07 May 2010 19:49:18 GMT

Procedure

←Older revision		Revision as of 19:49, 7 May 2010
Line 6:		Line 6:

	==Procedure==		==Procedure==
-	#~~Pretreat bacteria~~ with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase	+	#Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
-	#incubate at ~~37C~~ for 15-30 minutes	+	#incubate at 37°C for 15-30 minutes.
-	#~~Perform QIAGEN~~ miniprep	+	#Add Qiagen P2 buffer and continue miniprep as usual.
	#Verify plasmid existance by sequencing or running digests on gel		#Verify plasmid existance by sequencing or running digests on gel

Michael A. Speer: /* References */

Michael A. Speer — Fri, 07 May 2010 19:47:03 GMT

References

←Older revision		Revision as of 19:47, 7 May 2010
Line 22:		Line 22:
	'''Relevant papers and books'''		'''Relevant papers and books'''

-	Mathiesen et al, Journal of Applied Microbiology ~~2004~~	+	Mathiesen, G., H. M. Namlos, et al. (2004). "Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11." Journal of Applied Microbiology 96(4): 819-827.

	==Contact==		==Contact==

Torsten Waldminghaus at 17:12, 23 February 2009

Torsten Waldminghaus — Mon, 23 Feb 2009 17:12:44 GMT

←Older revision		Revision as of 17:12, 23 February 2009
Line 1:		Line 1:
-		+	{{back to protocols}}
	==Overview==		==Overview==

Derek Ju: /* Procedure */

Derek Ju — Thu, 30 Oct 2008 01:20:51 GMT

Procedure

←Older revision		Revision as of 01:20, 30 October 2008
Line 6:		Line 6:

	==Procedure==		==Procedure==
-	#~~Step 1~~	+	#Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
-	#~~Step 2~~	+	#incubate at 37C for 15-30 minutes
-	~~#*Step 2 has some additional information that goes with it. i.e. Keep~~ at ~~4°C.~~	+	#Perform QIAGEN miniprep
-	#~~Step 3~~	+	#Verify plasmid existance by sequencing or running digests on gel
-	#~~#Step 3 has multiple sub-steps within it.~~	+
-	~~##Enumerate each of those.~~	+

	==Notes==		==Notes==

Derek Ju: New page: ==Overview== Method to miniprep plasmid DNA from ''Lactobacillus plantarum'' ==Procedure== #Step 1 #Step 2 #*Step 2 has some additional information that goes with it. i.e. Keep at 4&d...

Derek Ju — Thu, 30 Oct 2008 01:19:23 GMT

New page: ==Overview== Method to miniprep plasmid DNA from ''Lactobacillus plantarum'' ==Procedure== #Step 1 #Step 2 #*Step 2 has some additional information that goes with it. i.e. Keep at 4&d...

New page

==Overview==

Method to miniprep plasmid DNA from ''Lactobacillus plantarum''

==Procedure==
#Step 1
#Step 2
#*Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
#Step 3
##Step 3 has multiple sub-steps within it.
##Enumerate each of those.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
#List troubleshooting tips here.
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

==References==
'''Relevant papers and books'''

Mathiesen et al, Journal of Applied Microbiology 2004

==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].

[[Category:Protocol]]

[[Category:In vitro]]

[[Category:Protein]]

[[Category:Lactobacillus]]