Lactobacillus planarum miniprep

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(New page: ==Overview== Method to miniprep plasmid DNA from ''Lactobacillus plantarum'' ==Procedure== #Step 1 #Step 2 #*Step 2 has some additional information that goes with it. i.e. Keep at 4&d...)
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==Procedure==
==Procedure==
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#Step 1
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#Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
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#Step 2
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#incubate at 37C for 15-30 minutes
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#*Step 2 has some additional information that goes with it.  i.e. Keep at 4°C.
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#Perform QIAGEN miniprep
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#Step 3
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#Verify plasmid existance by sequencing or running digests on gel
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##Step 3 has multiple sub-steps within it.
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##Enumerate each of those.
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==Notes==
==Notes==

Revision as of 21:20, 29 October 2008

Contents

Overview

Method to miniprep plasmid DNA from Lactobacillus plantarum


Procedure

  1. Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
  2. incubate at 37C for 15-30 minutes
  3. Perform QIAGEN miniprep
  4. Verify plasmid existance by sequencing or running digests on gel

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

Mathiesen et al, Journal of Applied Microbiology 2004

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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