http://openwetware.org/index.php?title=Lactobacillus_miniprep&feed=atom&action=history 2013-05-24T12:00:14Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Lactobacillus_miniprep&diff=339550&oldid=prev 2009-08-17T19:09:57Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:09, 17 August 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 16:</td> <td colspan="2" class="diff-lineno">Line 16:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Please sign your name to your note by adding &lt;font face=&quot;courier&quot;&gt;&lt;nowiki&gt;'''*~~~~''':&lt;/nowiki&gt;&lt;/font&gt; to the beginning of your tip.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Please sign your name to your note by adding &lt;font face=&quot;courier&quot;&gt;&lt;nowiki&gt;'''*~~~~''':&lt;/nowiki&gt;&lt;/font&gt; to the beginning of your tip.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[User:Kelly Drinkwater|Kelly Drinkwater]] 15:09, 17 August 2009 (EDT) Here's a [http://www1.qiagen.com/literature/render.aspx?id=467|user-generated protocol for B. subtilis] from the Qiagen website. It's the same as a normal miniprep except you add lysozyme with the buffer P1 to 1mg/mL and incubate at 37C for 10 min. There's nothing posted about how it performs.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> <!-- diff generator: internal 2013-05-24 12:00:14 --> </table>

Kelly Drinkwater http://openwetware.org/index.php?title=Lactobacillus_miniprep&diff=288226&oldid=prev 2009-02-23T17:12:22Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:12, 23 February 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{back to protocols}}</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A miniprep for ''Lactobacillus delbruckii'' (development in progress)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A miniprep for ''Lactobacillus delbruckii'' (development in progress)</div></td></tr> <!-- diff generator: internal 2013-05-24 12:00:14 --> </table>

Torsten Waldminghaus http://openwetware.org/index.php?title=Lactobacillus_miniprep&diff=256619&oldid=prev 2008-10-30T01:17:02Z

<p>New page: ==Overview== A miniprep for ''Lactobacillus delbruckii'' (development in progress) ==Procedure== #Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) wit...</p> <p><b>New page</b></p><div><br /> ==Overview==<br /> A miniprep for ''Lactobacillus delbruckii'' (development in progress)<br /> <br /> <br /> ==Procedure==<br /> #Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) with 10mg/ml lysozyme and 70U/ml mutanolysin.<br /> #Perform normal QIAGEN miniprep <br /> #verify extraction of plasmid by sequencing or digesting and running on gel<br /> <br /> ==Notes==<br /> Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!<br /> #List troubleshooting tips here. <br /> #You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.<br /> #Anecdotal observations that might be of use to others can also be posted here. <br /> <br /> Please sign your name to your note by adding &lt;font face=&quot;courier&quot;&gt;&lt;nowiki&gt;'''*~~~~''':&lt;/nowiki&gt;&lt;/font&gt; to the beginning of your tip.<br /> <br /> ==References==<br /> '''Relevant papers and books'''<br /> Serror et al, Applied and Environmental Microbiology, 2002<br /> <br /> ==Contact==<br /> *Derek Ju (derekju [at] mit.edu)<br /> <br /> or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br /> <br /> <br /> [[Category:Protocol]]<br /> <br /> [[Category:Lactobacillus]]<br /> <br /> [[Category:In vivo]]</div>

Derek Ju