Lactobacillus miniprep

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (14:09, 17 August 2009) (view source)
(Notes)
 
Line 16: Line 16:
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
 +
 +
*[[User:Kelly Drinkwater|Kelly Drinkwater]] 15:09, 17 August 2009 (EDT) Here's a [http://www1.qiagen.com/literature/render.aspx?id=467|user-generated protocol for B. subtilis] from the Qiagen website. It's the same as a normal miniprep except you add lysozyme with the buffer P1 to 1mg/mL and incubate at 37C for 10 min. There's nothing posted about how it performs.
==References==
==References==

Current revision

back to protocols

Contents

Overview

A miniprep for Lactobacillus delbruckii (development in progress)


Procedure

  1. Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) with 10mg/ml lysozyme and 70U/ml mutanolysin.
  2. Perform normal QIAGEN miniprep
  3. verify extraction of plasmid by sequencing or digesting and running on gel

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

  • Kelly Drinkwater 15:09, 17 August 2009 (EDT) Here's a protocol for B. subtilis from the Qiagen website. It's the same as a normal miniprep except you add lysozyme with the buffer P1 to 1mg/mL and incubate at 37C for 10 min. There's nothing posted about how it performs.

References

Relevant papers and books Serror et al, Applied and Environmental Microbiology, 2002

Contact

  • Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.

Personal tools