Lactobacillus compatibility

Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation

• VI104 strain-
  • -pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10^4) transformants/ug
  • - pX3 plasmid (from L. delbrueckii) (10^3)
  • - pJK650 plasmid (from L. delbrueckii) (10^3)
  • - pULP8 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
  • - pNZ12 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
  • - pG+ host 4 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
  • - pGB305 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
  • - pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
  • - pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
• ATCC 11842 strain –
  • -pJK650 plasmid (2 x 10^3)

Lactobacillus delbruckii subsp. lactis and Compatible Plasmid(s) for Electrotransformation

• ATCC 10697 strain -
  • -pLEM415 plasmid (10^3)
  • -pJK650 plasmid (10^3)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for compatible plasmids?? Look here: pJK650 and pLEM415

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for the transformation protocol? Look here: '''lactobacillus transformation'''

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

1. et al, Applied and Environmental Microbiology 2002

Contact

• Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.