LEDOMG:Equipment: Difference between revisions
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'''Constantin Sandu Aldea Alley, 10, 012065 Bucuresti, Romania, ledomg@lccsms.ro'''<br> | |||
'''Central Laboratory for Quality of Seed and Planting Material (CLQSPM) - Genetically Modified Organisms Detection Laboratory and Electrophoresis - LEDOMG'''<br> | |||
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[[LEDOMG | <font face="trebuchet ms" size=2 style="color:#ffffff"> '''HOME''' </font>]] | |||
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[[LEDOMG:Contact | <font face="trebuchet ms" size=2 style="color:#ffffff"> '''Contact''' </font>]] | |||
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[[LEDOMG:Protocols | <font face="trebuchet ms" size=2 style="color:#ffffff"> '''Protocols''' </font>]] | |||
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[[LEDOMG:Equipment | <font face="trebuchet ms" size=2 style="color:#ffffff"> '''Equipment''' </font>]] | |||
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*<strong>[https://www.erris.gov.ro/LEDOMG LEDOMG's Facility overview]</strong> Romanian Research Infrastructures System<br> | *<strong>[https://www.erris.gov.ro/LEDOMG LEDOMG's Facility overview]</strong> Romanian Research Infrastructures System<br> | ||
<br> | |||
Safety and quality control<br> | |||
[[Image:P1020295.JPG|thumb|left|alt=Grinding room, face and ear protection|3M6200, 3M501, 3M Peltor Optime I, H510A]]<br> | |||
3M™ Reusable Half Face Mask Respirator<br>PPE Safety Solutions 3M™ Filter Retainer 501<br>3M H510A Peltor Optime I Headband Ear Muff Defenders<br>3M 5925 P2 Filter<br><br><br> <br> <br> | |||
<br> BioPhometer plus 6132<br> | |||
[[Image:IMGP5512.JPG|thumb|left|alt=BioPhometer plus 6132|BioPhometer plus 6132]]<br> | |||
[[Image:Picture1-in-use-ad.jpg|thumb|right|alt=BioPhometer plus 6132|During opperation]] | |||
[[Image:Graph-dna-excel.png|thumb|left|alt=excel graph of data|Excel graph of data]]<br> | |||
*[[Media:Biophotometer-manual-old.pdf|Biophotometer manual, (old)]] source *[[http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf|//arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf]] | *[[Media:Biophotometer-manual-old.pdf|Biophotometer manual, (old)]] source *[[http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf|//arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf]] | ||
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*[[Media:Userguide_BioPhotometer_plus.pdf|Troubleshooting Guide for the measurement of nucleic acids with the BioPhotometer plus]] source *[[http://www.lhybride.info/media/file/Userguide_BioPhotometer_plus.pdf]] | *[[Media:Userguide_BioPhotometer_plus.pdf|Troubleshooting Guide for the measurement of nucleic acids with the BioPhotometer plus]] source *[[http://www.lhybride.info/media/file/Userguide_BioPhotometer_plus.pdf]] | ||
Cuvettes for BioPhometer plus 6132o | |||
*[[Media:Instructions-for-use_UVette.pdf|Eppendorf UVette® 220-1600 nm Cat. no.:952010051]] source*[[https://www.eppendorf.com/OC-en/downloads/OC-en/087160_Operating-Manual.pdf]] | |||
Generate lot specific QC certificate link *[[https://www.eppendorf.com/US-en/service-support/quality-regulatory-affairs/certificates/lot-specific-certificates/]] | |||
Useful documents on DNA Spectrophotometric analysis | |||
*[[Media:Unit4instructorsmanual.pdf|Genomic DNA Purification Instructor’s Manual]] source*[[https://www.promega.com/~/media/files/resources/education%20and%20training/unit%204/unit4instructorsmanual.pdf?la=en]] | *[[Media:Unit4instructorsmanual.pdf|Genomic DNA Purification Instructor’s Manual]] source*[[https://www.promega.com/~/media/files/resources/education%20and%20training/unit%204/unit4instructorsmanual.pdf?la=en]] | ||
*[[Media:T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf|T042‐TECHNICAL BULLETIN NanoDrop Spectrophotometers]] source *[[http://www.nanodrop.com/Library/T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf]] | *[[Media:T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf|T042‐TECHNICAL BULLETIN NanoDrop Spectrophotometers]] source *[[http://www.nanodrop.com/Library/T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf]] | ||
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*[[Media:5-clark.pdf|An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment William Clark and Kimberley Christopher Department of Biological Sciences University of Alberta Edmonton, Alberta, CANADA T6G 2E9]] source *[[http://www.ableweb.org/volumes/vol-22/5-clark.pdf]] | *[[Media:5-clark.pdf|An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment William Clark and Kimberley Christopher Department of Biological Sciences University of Alberta Edmonton, Alberta, CANADA T6G 2E9]] source *[[http://www.ableweb.org/volumes/vol-22/5-clark.pdf]] | ||
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Fluoroskan Ascent FL; Thermo Scientific | |||
*[[Media:Fluoroskan-Ascent-Ascent-FL-old-manual.pdf|Fluoroskan Ascent FL manual, (old)]] source *[http://www.stmichaelshospital.com/pdf/research/facilities-equipment/Fluoroskan-Ascent-Ascent%20FL.pdf] | |||
*[[Media:Fluoroskan-Ascent-Ascent-FL-manual.pdf|Fluoroskan Ascent FL manual]] source *[http://www.thermoscientific.com/content/dam/tfs/LPG/LCD/LCD%20Documents/Product%20Manuals%20&%20Specifications/Microplate%20Instrumentation/Microplate%20Readers/D01518~.pdf] | |||
*[[Media:Ascent_SW_User_Manual_for_Fluoroskan_Ascent%2C_CF_%26_FL.pdf|Fluoroskan Ascent FL user guide]] source *[http://dibernardo.tigem.it/internal-site/files/manual-fluoroskan/ascentsw.pdf] | |||
*[[Media:Detection_of_DNA_from_solution_with_PicoGreen.pdf|Detection of DNA from solution with PicoGreen]] source *[https://www.thermofisher.co.nz/Uploads/file/Scientific/Applications/Scientific-Instruments-Automation/Labsystems-PicoGreen-Application-Note.pdf] | |||
*[[Media:Detection_of_DNA_with_the_Thermo_Scientific.pdf|Detection of DNA with the Thermo Scientific]] source *[http://es.vwr-cmd.com/ex/downloads/life_science/biomarke/0709/bioMarkeMagazine-18_14.pdf] | |||
*[[Media:Fluoroskan_Ascent_FL_cat_page.pdf|Fluoroskan Ascent FL catalog]] source *[http://www.ninolab.se/fileadmin/Ninolab/pdf/thermolabsystems/Fluoroskan_Ascent_FL_cat_page.pdf] | |||
*[[Media:N_Chiu_Comparison_2005.pdf |Schiavo S, Yang W, Chiu NH and Krull IS. Comparison of fluorometric detection methods for quantitative PCR. Journal of Immunoassay & Immunochemistry, 26(1), 1-12 (2005) [copyright Taylor & Francis], available online at: http://www.tandfonline.com/10.1081/IAS-200041139]] source *[http://libres.uncg.edu/ir/uncg/f/N_Chiu_Comparison_2005.pdf] | |||
<br> | |||
Plates and reagents for Fluoroskan Ascent FL; Thermo Scientific | |||
*[[Media:QuantiFluor_dsDNA_System_Protocol.pdf|QuantiFluor® dsDNA E2670]] source *[https://www.promega.com/~/media/Files/Resources/Protocols/Technical%20Manuals/101/QuantiFluor%20dsDNA%20System%20Protocol.pdf] | |||
*[[Media:Black_96-Well_Immuno_Plates.pdf|Thermo Scientific Cliniplate 96-Well Microplates # 9502867 - Black Cliniplate, Polystyrene, Black, 50/cs]] source *[http://www.thermoscientific.com/en/product/black-96-well-immuno-plates.html] | |||
[[Image:FLOROMETRIE_OPERATIONAL.JPG|thumb|center|alt=BioPhometer plus 6132|Fluoroskan Ascent FL during opperation]] | |||
<br> <br> | |||
Fourtec; DataNet – Multi-point, online, wireless data monitoring system<br> | |||
[[Image:P1020693.JPG|thumb|left|alt=DNL808-BXT EXTERNAL TEMPERATURE with OMNICEK ER14505 and TADIRAN SL-760 DBOD 3.6 V AA Lithium battery|DNL808-BXT]] | |||
[[Image:P1020690.JPG|thumb|right|alt=DNR800 Receiver/Repeater|DNR800 Receiver]] | |||
[[Image:Senzori.jpg|thumb|left|alt=DataSuite|DataSuite]] | |||
[[Image:P1020694.JPG|thumb|right|alt=DNR900 Receiver/Repeater|DNR900 Receiver/Repeater]] | |||
*[[Media:DataSuite_v2.0.5.0_Installation_CD_Read_Me.pdf|DataSuite v2.0.5.0 Installation CD Read Me]] | |||
*[[Media:DataSuite_Installation_and_Registration_Guide.pdf|DataSuite Installation and Registration Guide]] | |||
*[[Media:Report_14.06.2016_07.40.07.pdf|Report_14.06.2016_07.40.07.pdf]] | |||
*[[Media:Statistics_Report.pdf|Statistics Report one device]] | |||
*[[Media:DataSuite__Table.pdf|DataSuite Table one device]] | |||
*[[Media:DataSuite__Graph.pdf|DataSuite Graph one device]] | |||
*[[Media:Graph_excel_export.xlsx|DataSuite Graph excel export one device]] | |||
*[[Media:Datasuite_setup.pdf|DataSuite setup – print screen]] | |||
*[[Media:SL-760.pdf|SL-760 – TESTED 3.6 V AA Lithium battery 1 for DNL808-BXT EXTERNAL TEMPERATURE]] | |||
*[[Media:Range_of_products_er14505.pdf|ER14505 – TESTED 3.6 V AA Lithium battery 2 for DNL808-BXT EXTERNAL TEMPERATURE]] |
Latest revision as of 03:44, 28 November 2023
Constantin Sandu Aldea Alley, 10, 012065 Bucuresti, Romania, ledomg@lccsms.ro |
|
- LEDOMG's Facility overview Romanian Research Infrastructures System
Safety and quality control
3M™ Reusable Half Face Mask Respirator
PPE Safety Solutions 3M™ Filter Retainer 501
3M H510A Peltor Optime I Headband Ear Muff Defenders
3M 5925 P2 Filter
BioPhometer plus 6132
- Biophotometer manual, (old) source *[[1]]
- BioPhometer plus 6132 manual source *[[2]]
- Troubleshooting Guide for the measurement of nucleic acids with the BioPhotometer plus source *[[3]]
Cuvettes for BioPhometer plus 6132o
Generate lot specific QC certificate link *[[5]]
Useful documents on DNA Spectrophotometric analysis
- Genomic DNA Purification Instructor’s Manual source*[[6]]
- T042‐TECHNICAL BULLETIN NanoDrop Spectrophotometers source *[[7]]
- Understanding and measuring variations in DNA sample quality source *[[8]]
- An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment William Clark and Kimberley Christopher Department of Biological Sciences University of Alberta Edmonton, Alberta, CANADA T6G 2E9 source *[[9]]
Fluoroskan Ascent FL; Thermo Scientific
- Fluoroskan Ascent FL manual, (old) source *[10]
- Fluoroskan Ascent FL manual source *[11]
- Fluoroskan Ascent FL user guide source *[12]
- Detection of DNA from solution with PicoGreen source *[13]
- Detection of DNA with the Thermo Scientific source *[14]
- Fluoroskan Ascent FL catalog source *[15]
- Schiavo S, Yang W, Chiu NH and Krull IS. Comparison of fluorometric detection methods for quantitative PCR. Journal of Immunoassay & Immunochemistry, 26(1), 1-12 (2005) [copyright Taylor & Francis], available online at: http://www.tandfonline.com/10.1081/IAS-200041139 source *[16]
Plates and reagents for Fluoroskan Ascent FL; Thermo Scientific
- QuantiFluor® dsDNA E2670 source *[17]
- Thermo Scientific Cliniplate 96-Well Microplates # 9502867 - Black Cliniplate, Polystyrene, Black, 50/cs source *[18]
Fourtec; DataNet – Multi-point, online, wireless data monitoring system
- DataSuite v2.0.5.0 Installation CD Read Me
- DataSuite Installation and Registration Guide
- Report_14.06.2016_07.40.07.pdf
- Statistics Report one device
- DataSuite Table one device
- DataSuite Graph one device
- DataSuite Graph excel export one device
- DataSuite setup – print screen
- SL-760 – TESTED 3.6 V AA Lithium battery 1 for DNL808-BXT EXTERNAL TEMPERATURE
- ER14505 – TESTED 3.6 V AA Lithium battery 2 for DNL808-BXT EXTERNAL TEMPERATURE