LB

Summary

L-Broth or LB Medium. Standard growth medium for a variety of bacteria and conditions.

Ingredients

1. 10 g Bacto-tryptone
2. 5 g yeast extract
3. 10 g NaCl
4. distilled water

Protocol

1. Mix dry ingredients and add distilled water up to 1 Liter
2. Pour into 2 L flask (or greater)
3. Autoclave (liquid cycle)
   • 250°F, 22psi, 30 minutes

Source

J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001)