LB: Difference between revisions
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#5 g yeast extract | #5 g yeast extract | ||
#10 g NaCl | #10 g NaCl | ||
==Protocol== | ==Protocol== | ||
#Mix dry ingredients and add distilled water up to 1 Liter | #Mix dry ingredients and add distilled water up to 1 Liter | ||
| Line 12: | Line 11: | ||
#* 250°'''F''', 22psi, 30 minutes | #* 250°'''F''', 22psi, 30 minutes | ||
==Source== | ==Source== | ||
J. Sambrook, D.W. Russell, ''Molecular Cloning: A Laboratory Manual'' (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) | Adapted From: | ||
J. Sambrook, D.W. Russell, ''Molecular Cloning: A Laboratory Manual'' (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2 | |||
Revision as of 13:05, 3 June 2005
Summary
L-Broth or LB Medium. Standard growth medium for a variety of bacteria and conditions.
Ingredients
- 10 g Bacto-tryptone
- 5 g yeast extract
- 10 g NaCl
Protocol
- Mix dry ingredients and add distilled water up to 1 Liter
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- 250°F, 22psi, 30 minutes
Source
Adapted From:
J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2
