LB: Difference between revisions
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== Summary == | == Summary == | ||
Luria-Bertani Medium (aka L-Broth or LB Medium). | Luria-Bertani Medium (aka L-Broth or LB Medium). (Bertani says LB really stands for lysogeny broth.) LB is a standard growth medium for a variety of bacteria and conditions. | ||
== Ingredients == | == Ingredients == | ||
#10 g Bacto-tryptone | #10 g Bacto-tryptone | ||
#5 g yeast extract | #5 g yeast extract | ||
| Line 7: | Line 9: | ||
See also: [[Silver: LB Liquid]] | See also: [[Silver: LB Liquid]] | ||
''Note:'' There are two formulations of LB, Miller and Lennox, that differ in the amount of NaCl. Lennox has less salt, only 5 g/L. The Qiagen miniprep kit recommends LB with 10 g NaCl for highest plasmid yields. | |||
==Protocol== | ==Protocol== | ||
#Mix dry ingredients and add distilled water up to 1 Liter | #Mix dry ingredients and add distilled water up to 1 Liter | ||
Revision as of 12:27, 15 April 2006
Summary
Luria-Bertani Medium (aka L-Broth or LB Medium). (Bertani says LB really stands for lysogeny broth.) LB is a standard growth medium for a variety of bacteria and conditions.
Ingredients
- 10 g Bacto-tryptone
- 5 g yeast extract
- 10 g NaCl
See also: Silver: LB Liquid
Note: There are two formulations of LB, Miller and Lennox, that differ in the amount of NaCl. Lennox has less salt, only 5 g/L. The Qiagen miniprep kit recommends LB with 10 g NaCl for highest plasmid yields.
Protocol
- Mix dry ingredients and add distilled water up to 1 Liter
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- 250°F, 22psi, 30 minutes
Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0 with 5M NaOH (~200µL). We usually obtain this from the kitchen.
Source
Adapted From:
J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2
