Kubke Lab:Research/CND/Records2010-2011Summer/MH007: Difference between revisions
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=Embryo details= | =Embryo details= | ||
[[Image: MH007 Fixed and Stored in a Vial.JPG|thumb|right]] | |||
<br>'''Species''': Gallus gallus domesticus | <br>'''Species''': Gallus gallus domesticus | ||
<br>'''Embryo Name''': MH007 | <br>'''Embryo Name''': MH007 | ||
<br>'''Embryo stage''': ST25 Confirmed by Fabiana (supervisor). | <br>'''Embryo stage''': ST25 Confirmed by Fabiana (supervisor).[[Image:MH007 ST25.JPG|thumb|left]] | ||
<br>'''Staging description''': | <br>'''Staging description''': | ||
<br>'''Fixation''': PFA | <br>'''Fixation''': PFA | ||
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<br>'''Objective''': | <br>'''Objective''': | ||
<br>'''Procedure''': | <br>'''Procedure''': | ||
[[Image: MH007 Cut at wingbud.JPG|thumb|right]] | |||
[[Image:MH007 in mould.JPG|thumb|right]] | |||
[[Image:MH007 in mould large.JPG|thumb|right]] | |||
[[Image:MH007 in block ready to cut.JPG|thumb|right]] | |||
[[Image: MH007 in trimmed block.JPG|thumb|right]] | |||
<br>'''Comments''': | <br>'''Comments''': | ||
Revision as of 18:32, 6 February 2011
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: MH007
Embryo stage: ST25 Confirmed by Fabiana (supervisor).
Staging description:
Fixation: PFA
Cryoprotection: None
Material label and storage:
Experiment details
Objective:
Procedure:
Comments:
To use this template copy and paste the following text:
Cryostat Sectioning
Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)
| Cryostat | |
| Knife | |
| Day Cut | |
| Knife Angle | |
| Chamber Temp | |
| Object Temp | |
| Glass Slides | |
| Plane of section | |
| Number of slides | |
| Observations |
(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)
Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol
| Date | ||
| Defatting and rehydration step | ||
| Solution | Time | Comments |
| Water | ||
| 75% alcohol | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Xylene 2 | ||
| Xylene 1 | ||
| 100% alcohol 2 | ||
| 100% alcohol 1 | ||
| 95% alcohol | ||
| 75% alcohol | ||
| Water | ||
| Staining and differentiation step | ||
| Solution | Time | Comments |
| Water | ||
| Cresyl Violet | ||
| 50% alcohol | ||
| 70% alcohol acetic acid | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Coverslip | ||
