Kubke Lab:/Notebook/Cranial nerve development/2014/11/25
Cranial Nerve Development | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleGeneralHelenMy dyes finally worked today! I saw the whole process through and whole mounted them etc and seemed to get a really clear and flat image on the Leica dissecting microscope. Fabiana and I were surprised at how clear and magnified the images could get on the Leica which is really great! The dye went really well and we got a full coverage injection where I could see the afferents extend to the fullest that they do and the efferent s turn clearly. Tomorrow I want to inject two different colours on the same nerve but on different sides to see whether the nerves decussate and intermingle with each other. I cracked eggs that had been in the small incubator today for 4 days and they were luckily all alive however at very small stages- stage 15 which you would not expect for a 4 day old embryo! I have left one and I will crack it open tomorrow to see if it has grown and how long it takes for these eggs to get to the stage that I want them at in the little incubator. Fabiana |