Koeris/Notebook/2007-1-21: Difference between revisions
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===Pipetting Scheme=== | ===Pipetting Scheme=== | ||
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! No antibiotic !! No antibiotic !! No antibiotic !! No antibiotic !! Kanamycin 30 ug/ml !! Kanamycin 30 ug/ml | |||
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! wt LB !! wt M9 !! DhipBA LB !! DhipBA M9 !! DhipA LB !! DhipA M9 | |||
|- | |||
! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution | |||
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Revision as of 09:21, 24 January 2007
hipA assay
Growth conditions
Grew all samples from previous 16h ON in
- 50ml LB + Cam
or
- 50ml M9 + 0.2% glucose + Cam
for another 16h, at 37 deg C and 300rpm shaking
Persister assay
Pipetting Scheme
| No antibiotic | No antibiotic | No antibiotic | No antibiotic | Kanamycin 30 ug/ml | Kanamycin 30 ug/ml |
|---|---|---|---|---|---|
| wt LB | wt M9 | DhipBA LB | DhipBA M9 | DhipA LB | DhipA M9 |
| Serial dilution | Serial dilution | Serial dilution | Serial dilution | Serial dilution | Serial dilution |
