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Kinesin / Microtubule Molecular Motor System
Effects of casein surface passivation in kinesin-1 gliding motility assay
Maloney A, Herskowitz LJ, Koch SJ (2011) Effects of Surface Passivation on Gliding Motility Assays. PLoS ONE 6(6): e19522. http://dx.doi.org/10.1371/journal.pone.0019522
Andy Maloney studies the effects of alpha, beta, kappa, and whole casein in the kinesin-1 gliding motility assay. Automated speed analysis software by Larry Herskowitz.
Figure: Black circles are alpha casein passivation, red squares are beta casein passivation, green up pointing triangles are whole casein passivation, and blue down pointing triangles are mixed casein passivation. Each data point is the mean from three different samples, taken at approximately the same assay time. Error bars represent the standard error of the mean. Alpha casein had the most consistent average speed measurements at 949±4 nm/s. Whole casein and mixed casein averaged to 966±7 nm/s and 966±6 nm/s respectively. Beta casein averaged to 870±30 nm/s.
A discrete-state model for kinesin-1 with rate constants modulated by neck linker tension (under review, PLoS ONE)
Koch, Steven and Herskowitz, Lawrence. A Discrete State Model for Kinesin-1 with Rate Constants Modulated by Neck Linker Tension. Available from Nature Precedings http://hdl.handle.net/10101/npre.2010.5038.1 (2010)
Effect of water isotope (deuterium or oxygen-18) on the kinesin-1 gliding motility assay (in preparation)
Andy Maloney took painstakingly clean data on how D2O and oxygen-18 water affect the gliding speed in the kinesin-1 gliding motility assay. We are preparing this data for submission to PLoS ONE. We are doing further investigation to understand what the mechanism is and whether it is an interesting way to probe this and other biomolecular systems. The effect is strikingly similar to the change in viscosity of the solvents, but we have not yet proven that is the mechanism.
See Andy Maloney's dissertation: http://www.openwetware.org/wiki/User:Andy_Maloney/Water_isotope_effects_on_kinesin_and_microtubules
Open Source Kinesin-1 Stochastic Simulation and Gliding Assay Tracking Software (pre-print only)
Herskowitz, Lawrence, Salvagno, Anthony, Josey, Brian, Maloney, Andy, and Koch, Steven. Open Source Kinesin-1 Stochastic Simulation and Gliding Assay Tracking Software. Available from Nature Precedings http://dx.doi.org/10.1038/npre.2010.4468.1 (2010)
Larry Herskowitz wrote this software to test his microtubule image tracking. It's a nice LabVIEW application for generating a series of images that simulate microtubules fractionally-labeled with dye molecules. You can design a route for the microtubules to follow. We are happy to provide the source code and help you get it running on your own system. We decided that it was not worth peer reviewers' time to submit this for formal publication, as we did not anticipate any benefits besides padding our resumes. So, we will leave it at the preprint stage on Nature Precedings.
In George Bachand's lab with Susan Rivera, fungal kinesin from Thermomyces lanuginosus
Rivera SB, Koch SJ, Bauer JM, Edwards JM, Bachand GD. 2007. "Temperature dependent properties of a kinesin-3 motor protein from Thermomyces lanuginosus." Fungal Genetics and Biology 44:1170-1179. PMID 17398126
Figure: Dynamic light scattering data showing the inhibition by ATP of heat-induced aggregation of kinesin from Thermomyces lanuginosus.