Koch Lab:Publications: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 16: | Line 16: | ||
[[Image:PN 186 sensor 45deg 08a big.PNG|right|thumb|Scanning Electron Microscope image of a force sensor similar that used for the research.]] | [[Image:PN 186 sensor 45deg 08a big.PNG|right|thumb|Scanning Electron Microscope image of a force sensor similar that used for the research.]] | ||
[http://link.aip.org/link/?APL/89/173901 Koch SJ, Thayer GE, Corwin AD, de Boer MP. Micromachined piconewton force sensor for biophysics investigations. Appl. Phys. Let. 2006 Oct 23;89(17):173901] ([http://www.chtm.unm.edu/publications/APL%2089_173901_Koch,%20Thayer,%20Corwin,%20de%20Boer_MEMS%20force%20sensor%20for%20mag%20bead%20calibration.pdf PDF]) | [http://link.aip.org/link/?APL/89/173901 Koch SJ, Thayer GE, Corwin AD, de Boer MP. Micromachined piconewton force sensor for biophysics investigations. Appl. Phys. Let. 2006 Oct 23;89(17):173901] ([http://www.chtm.unm.edu/publications/APL%2089_173901_Koch,%20Thayer,%20Corwin,%20de%20Boer_MEMS%20force%20sensor%20for%20mag%20bead%20calibration.pdf PDF]) | ||
==[[/Drafts|Paper Drafts]]== | |||
<br> | <br> | ||
<br> | <br> |
Revision as of 16:21, 1 March 2008
Home | Research | Lab Members | Publications | Protocols | Contact | Funding |
Principles | Data | Notebooks | Links | Meetings | Presentations | Inventory |
Probing Protein-DNA Interactions by Unzipping DNA with Optical Tweezers
Our initial proof-of-principle publication in the Wang Lab at Cornell
Koch SJ, Shundrovsky A, Jantzen BC, Wang MD. Probing protein-DNA interactions by unzipping a single DNA double helix. Biophys J. 2002 Aug;83(2):1098-105. PMID 12124289
Our follow-on paper showing that unbinding forces can be analyzed nicely with Evan Evans' Dynamic Force Spectrosocpy (DFS) model
Koch SJ, Wang MD. Dynamic force spectroscopy of protein-DNA interactions by unzipping DNA. Phys Rev Lett. 2003 Jul 11;91(2):028103. PMID 12906513
- Buried in this paper is the "loading rate clamp" that we used and which greatly simplifies data analysis as well as provides much cleaner data. Also, our maximum likelihood method for data analysis is better than the typical method of fitting Gaussians to histograms, but this was also buried in footnotes. It's been while since published, but the Koch lab would like to publish the details of these methods, as they would be very helpful to others doing DFS.
MEMS Force Sensor for Biophysics
Work done with Gayle Thayer, Alex Corwin, Maarten de Boer at Sandia, finished up after Koch moved to UNM, Physics & CHTM
Koch SJ, Thayer GE, Corwin AD, de Boer MP. Micromachined piconewton force sensor for biophysics investigations. Appl. Phys. Let. 2006 Oct 23;89(17):173901 (PDF)
Paper Drafts