Koch Lab:Protocols/Kinesin: Difference between revisions
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[http://www.proweb.org/kinesin/Methods/ The Kinesin Home Page] is an excellent resource. Many of our protocols probably evolved from there, which in turn were obtained from prior work of others. It's tough to know who to cite on these protocols, so please send us a note if you'd like us to cite it properly. | [http://www.proweb.org/kinesin/Methods/ The Kinesin Home Page] is an excellent resource. Many of our protocols probably evolved from there, which in turn were obtained from prior work of others. It's tough to know who to cite on these protocols, so please send us a note if you'd like us to cite it properly. | ||
==Chemicals== | |||
* [[/ATP suspension|ATP suspension]] | |||
* [[/GTP suspension|GTP suspension]] | |||
* [[/Taxol suspension|Taxol suspension]] | |||
* [[/Glycerol|Glycerol]] | |||
* [[/kappa-Casein|<math>\kappa</math>-Casein]] | |||
* [[/PIPES|PIPES]] | |||
* [[/EGTA|EGTA]] | |||
* [[/MgCl2|MgCl<sub>2</sub>]] | |||
* [[/NaOH|NaOH]] | |||
==Buffers== | ==Buffers== | ||
* [[/BRB80 Acid Pipes| | * [[/PEM|PEM]] | ||
** [[/BRB80 Acid Pipes|Koch's version of PEM]] | |||
:: [[User:Steven J. Koch|Steve Koch]]: I can't remember whether I made this buffer for use with malachite green inorganic phosphate assays, or because I thought it was better to use potassium ions versus sodium ions in kinesin assays. | |||
* [[/Tubulin storage buffer|Tubulin storage buffer]] | |||
:: Used to store as well as polymerize tubulin. | |||
* [[/PEM-T|PEM-T]] | |||
:: Used to stabilize microtubules after polymerization. | |||
* [[/PEM-kappaC|PEM-<math>\kappa</math>C]] | |||
:: Used to passivate glass in order to keep kinesin viable. | |||
==Methods== | ==Methods== | ||
* [[/Tubulin resuspension and polymerization|Tubulin resuspension and polymerization (Cytoskeleton)]] | |||
* [[/AMP-PNP Purification|AMP-PNP Purification of active kinesin]] | * [[/AMP-PNP Purification|AMP-PNP Purification of active kinesin]] | ||
* [[/DLS Kinesin Aggregation|Dynamic Light Scattering measurement of kinesin aggregation]] | |||
Latest revision as of 18:51, 30 September 2009
The Kinesin Home Page is an excellent resource. Many of our protocols probably evolved from there, which in turn were obtained from prior work of others. It's tough to know who to cite on these protocols, so please send us a note if you'd like us to cite it properly.
Chemicals
- ATP suspension
- GTP suspension
- Taxol suspension
- Glycerol
- [math]\displaystyle{ \kappa }[/math]-Casein
- PIPES
- EGTA
- MgCl2
- NaOH
Buffers
- Steve Koch: I can't remember whether I made this buffer for use with malachite green inorganic phosphate assays, or because I thought it was better to use potassium ions versus sodium ions in kinesin assays.
- Used to store as well as polymerize tubulin.
- Used to stabilize microtubules after polymerization.
- Used to passivate glass in order to keep kinesin viable.
