Koch Lab:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Old Page to be deleted)
Current revision (13:40, 24 January 2011) (view source)
(General Lab Techniques)
 
(4 intermediate revisions not shown.)
Line 9: Line 9:
*[[/How to Clean Glassware|Cleaning Glassware]]
*[[/How to Clean Glassware|Cleaning Glassware]]
*[[/How to use a pipette|Using Pipettes]]
*[[/How to use a pipette|Using Pipettes]]
 +
*[[/Changing the Hg bulb|Changing the Hg bulb]]
*[[/Laser Safety|Laser Safety]]
*[[/Laser Safety|Laser Safety]]
*[[/How to Autoclave|How to Autoclave]]
*[[/How to Autoclave|How to Autoclave]]
 +
*[[/How to Clean the Autoclave|How to Clean the Autoclave]]
 +
*[[/Recycling|Recycling]]
==Molecular Biology Protocols==
==Molecular Biology Protocols==
===Standard Techniques===
===Standard Techniques===
*PCR
*PCR
-
*Gel Electrophoresis
+
*[[User:Anthony Salvagno/Notebook/Research/Notes About Gels|Gel Electrophoresis]]
*Cloning
*Cloning
*Transformation into E. Coli
*Transformation into E. Coli
Line 29: Line 32:
*[[/Gel Imaging System|Gel Imaging System]]
*[[/Gel Imaging System|Gel Imaging System]]
-
==[[/Computational Protocols|Computational Protocols]]==
+
==Computational Protocols==
*Shotgun DNA Mapping
*Shotgun DNA Mapping
*Kinesin Processivity
*Kinesin Processivity
Line 35: Line 38:
*Motion Detection Camera
*Motion Detection Camera
*[[/Loading rate clamp|Loading Rate Clamp]]
*[[/Loading rate clamp|Loading Rate Clamp]]
 +
* Maximum likelihood anlaysis of single-molecule disruption data with Evan Evans' et al. Dynamic Force Spectroscopy (DFS) model.  Much better and easier than performing Gaussian fits to histograms.  Used in 2003 Phys. Rev. Let. paper, PMID 12906513.
 +
* Finite Element Magnetic Modeling (FEMM) for predicting forces on magnetic microspheres, as in [http://link.aip.org/link/?APL/89/173901 2006 Appl. Phys. Let.] ([http://www.chtm.unm.edu/publications/APL%2089_173901_Koch,%20Thayer,%20Corwin,%20de%20Boer_MEMS%20force%20sensor%20for%20mag%20bead%20calibration.pdf PDF])
</div>
</div>

Current revision

This page is under construction.


Home Research Lab Members Publications Protocols Contact Funding
Principles Data Notebooks Links Meetings Presentations Inventory

One of the goals of our lab is to share protocols in a form that makes it as easy as possible for other labs to build off of them. These will be a combination of protocols that we will develop and also those that Steve has developed in the past but has not had a good opportunity to distribute yet. If any of the following protocols are of particular interest, drop us a line or make a note on the discussion page! We also want to share software applications and computational modules (mostly LabVIEW) that would be useful to others.

Contents

General Lab Techniques

Every lab has its own standards when it relates to lab safety, cleanliness, and shared equipment protocols. Below we've documented our specific techniques with regards to these general lab standards:

Molecular Biology Protocols

Standard Techniques

KochLab Techniques

Equipment Setup

Computational Protocols

  • Shotgun DNA Mapping
  • Kinesin Processivity
  • Kinesin Tracking
  • Motion Detection Camera
  • Loading Rate Clamp
  • Maximum likelihood anlaysis of single-molecule disruption data with Evan Evans' et al. Dynamic Force Spectroscopy (DFS) model. Much better and easier than performing Gaussian fits to histograms. Used in 2003 Phys. Rev. Let. paper, PMID 12906513.
  • Finite Element Magnetic Modeling (FEMM) for predicting forces on magnetic microspheres, as in 2006 Appl. Phys. Let. (PDF)


Personal tools