Koch Lab:Inventory/BRB80 (PEM): Difference between revisions

Latest revision as of 11:20, 28 August 2009

Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9

This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
About 205ml were created\
Concentrations:
- 1mM MgCl₂
- 1mM EGTA
- 80mM PIPES
- 12.5mM NaOH
New batch made 8/28/2009
Just over 200mL created
- 80mM PIPES
- 1mM EGTA
- 1mM MgCl₂
- 12.9mM NaOH

@@ Line 1: / Line 1: @@
 {{KL VWR|s=left}}
-==Koch Lab Buffer==
+==BRB80 or PEM (PIPES, EGTA, Magnesium) buffer==
-*This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
+Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9
+*This is the page for the buffer that Andy, Brigette and Linh made on [[User:Linh N Le/Notebook/2009/06/22|19:07, 22 June 2009 (EDT)]]
 *About 205ml were created\
 *Concentrations:
@@ Line 8: / Line 10: @@
 **1mM EGTA
 **80mM PIPES
-**125mM NaOH
+**12.5mM NaOH
+*New batch made [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/08/28#PEM 8/28/2009]
+*Just over 200mL created
+**80mM PIPES
+**1mM EGTA
+**1mM MgCl<sub>2</sub>
+**12.9mM NaOH
+===References===
+* [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe]
+* [http://openwetware.org/wiki/Koch_Lab:Protocols/Kinesin/BRB80_Acid_Pipes The actual recipe Steve used at Sandia]
+* [http://www.proweb.org/kinesin/Methods/motility.html Recipe on kinesin home page] (almost the same as cytoskeleton, but slightly lower pH)
+* [http://friendfeed.com/the-life-scientists/59d2ea57/does-anyone-here-know-what-initials-brb80-stand FriendFeed thread: what does the acronym BRB80 stand for?]