Knight:TOPO TA cloning

From OpenWetWare

Revision as of 16:30, 29 September 2005 by JeremyZucker (Talk | contribs)
Jump to: navigation, search

Notes

  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time ... even using day old PCR product can reduce efficiency.)
  • Your primers should NOT have 5' phosphates. They interfere with topoisomerase I.

Resources

Personal tools