Knight:TOPO TA cloning

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Revision as of 12:36, 29 September 2005 by Reshma P. Shetty (Talk | contribs)
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Notes

  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).

Resources

Retrieved from "http://openwetware.org/wiki/Knight:TOPO_TA_cloning"
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