Knight:TOPO TA cloning

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(Notes)
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*[[Tom Knight]] claims you can cut the reaction volume in half and it will still work fine.
*[[Tom Knight]] claims you can cut the reaction volume in half and it will still work fine.
*Use fresh PCR product for best results.  (The A overhang tends to get chewed back over time).
*Use fresh PCR product for best results.  (The A overhang tends to get chewed back over time).
 +
*Your primers should NOT have 5' phosphates.  They interfere with topoisomerase I.
==Resources==
==Resources==

Revision as of 16:30, 29 September 2005

Notes

  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).
  • Your primers should NOT have 5' phosphates. They interfere with topoisomerase I.

Resources

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