Knight:TOPO TA cloning: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Notes== | |||
*[[Tom Knight]] claims you can cut the reaction volume in half and it will still work fine. | |||
*Use fresh PCR product for best results. (The A overhang tends to get chewed back over time). | |||
==Resources== | ==Resources== | ||
*[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website] | *[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website] | ||
Revision as of 08:03, 29 September 2005
Notes
- Tom Knight claims you can cut the reaction volume in half and it will still work fine.
- Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).
