Knight:TOPO TA cloning
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| + | ==Notes== | ||
| + | *[[Tom Knight]] claims you can cut the reaction volume in half and it will still work fine. | ||
| + | *Use fresh PCR product for best results. (The A overhang tends to get chewed back over time). | ||
| + | |||
==Resources== | ==Resources== | ||
*[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website] | *[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website] | ||
Revision as of 11:03, 29 September 2005
Notes
- Tom Knight claims you can cut the reaction volume in half and it will still work fine.
- Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).
