Knight:Reconstituting primers - Revision history

Reshma P. Shetty at 14:12, 7 May 2010

2010-05-07T14:12:55Z

←Older revision		Revision as of 14:12, 7 May 2010
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	===Long version===		===Long version===
-	<math>Y\ \mu L\ =\ \frac{1}{25\ \mu L}X\ nmoles\ primer\ =\ \frac{110^6\ \mu M}{25000\ nmoles}X\ nmoles\ primer\ =\ 40\ \ X\ nmoles\ primer</math>	+	<math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}X\ nmoles\ primer\ =\ \frac{110^6\ \mu L}{25000\ nmoles}X\ nmoles\ primer\ =\ 40\ \ X\ nmoles\ primer</math>

	==Notes==		==Notes==

Reshma P. Shetty: /* Notes */

2007-03-14T15:34:03Z

Notes

←Older revision		Revision as of 15:34, 14 March 2007
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	See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.		See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.

-	[[Category:Protocol]] [[Category:DNA]]	+	[[Category:Protocol]] [[Category:DNA]] [[Category:In vitro]]

Reshma P. Shetty at 15:33, 14 March 2007

2007-03-14T15:33:42Z

←Older revision		Revision as of 15:33, 14 March 2007
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	==Notes==		==Notes==
	See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.		See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.
		+
		+	[[Category:Protocol]] [[Category:DNA]]

Reshma P. Shetty at 19:56, 26 May 2006

2006-05-26T19:56:29Z

New page

==Procedure==
Invitrogen recommends the following [http://www.invitrogen.com/content.cfm?pageid=9974 reconstitution procedure] -

#Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
#To make a 25 μM stock, add YμL of sterile H<sub>2</sub>O to X nmoles of dry primer stock. (See equations below).
#Allow to sit for 2 mins, then vortex for 15 secs.

===Short version===
<math>Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer</math>

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

===Long version===
<math>Y\ \mu L\ =\ \frac{1}{25\ \mu L}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu M}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>

==Notes==
See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.