Knight:Reconstituting primers

(Difference between revisions)

Current revision

Invitrogen recommends the following reconstitution procedure -

Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
To make a 25 μM stock, add YμL of sterile H₂O to X nmoles of dry primer stock. (See equations below).
Allow to sit for 2 mins, then vortex for 15 secs.

$Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer$

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

$Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer$

See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.

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 ===Long version===
-<math>Y\ \mu L\ =\ \frac{1}{25\ \mu L}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu M}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
+<math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
 ==Notes==
 See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.
-[[Category:Protocol]] [[Category:DNA]]
+[[Category:Protocol]] [[Category:DNA]] [[Category:In vitro]]