Knight:RNA electrophoresis

From OpenWetWare

Overview

Electrophoresis permits assessment of RNA by size and amount. In general, electrophoresis of RNA is done as a step prior to Northern analysis. However, this protocol is for visualizing the RNA in the gel.

Specific protocols

1. Knight:RNA electrophoresis/Denaturing
2. Knight:RNA electrophoresis/Native

Notes

• Avoid RNase contamination

References

1. Agarose Gel Electrophoresis of RNA [Ambion]