Knight:Protocols: Difference between revisions
(→DNA) |
|||
| Line 70: | Line 70: | ||
==Miscellaneous== | ==Miscellaneous== | ||
[[Knight:Deionization]] | |||
[[Knight:Dishwashing]] | [[Knight:Dishwashing]] | ||
Revision as of 18:03, 12 December 2006
<html><style type='text/css'> .tabs {
width: 750px; font-family: trebuchet ms;
}
.tabs strong{
color: #602;
} </style></html>
|
Knight Lab
|
DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Knight:Annealing and primer extension with Taq polymerase
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Knight:Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
RNA
Knight:In vitro transcription --in progress!
Knight:RNA electrophoresis --in progress!
Protein
Knight:Dialysis --works poorly
Knight:Electrophoretic mobility shift assay
Knight:Microcon filtration of proteins --works poorly
Knight:Purification of His-tagged proteins
Escherichia coli
Preparing chemically competent cells (Inoue)
Transforming chemically competent cells (Inoue)
Knight:Preparing electrocompetent cells
