Knight:Protocols: Difference between revisions
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[[Knight:Site-directed mutagenesis]] | [[Knight:Site-directed mutagenesis]] | ||
==''Escherichia coli''== | |||
[[Knight:Preparing electrocompetent cells]] |
Revision as of 10:28, 3 December 2005
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DNA
Annealing and primer extension
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Site-directed mutagenesis