Knight:Protein DNA binding

Overview

Various buffers for protein DNA binding reactions prior to gel shift assays.

Specific protocols/recipes

1. Knight:Protein DNA binding/Option 1
   • compiled from various Pabo lab papers
   • originally designed by Greisman to replication mammalian nuclear enviroment (Carl Pabo, personal communication)
2. Knight:Protein DNA binding/Option 2 -- in progress!
   • from recent Barbas lab papers
3. Knight:Protein DNA binding/Option 3 -- in progress!
   • simplified buffer recipe derived from above