Knight:Preparing stabs: Difference between revisions
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(New page: #Fill airtight, autoclavable vials with rubber or teflon caps 2/3 full of stab agar #Inoculate with a single colony repeatedly poking the inoculating loop into the agar #Incubate at 37°...) |
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Revision as of 06:37, 2 July 2008
- Fill airtight, autoclavable vials with rubber or teflon caps 2/3 full of stab agar
- Inoculate with a single colony repeatedly poking the inoculating loop into the agar
- Incubate at 37°C for 8-12 hrs until cloudy tracks of bacteria become visible
- Seal tightly and store in the dark at room temperature (15-22°C)
- Revive by using inoculating loop to streak onto a new LB plate
