Knight:Orders: Difference between revisions

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Knight Lab

Orders will be placed every Thursday unless otherwise requested.

Needs to be Ordered

96-deepwell plates (for DeLong MP robot)
Teknova plates
petri plate labels (for QC)
Acinetobacter baylyi ATCC 33305

Placed Orders

NEB streptavidin magnetic beads S1420S
ReqID 11106769
- Fireboy gas canisters (VWR 17919-890)
- adhesive foil covers (VWR 60941-076)
- LB broth
ReqID: 11105446
- Invitrogen PCR Supermix High Fidelity (Invitrogen 10790-020)

Received Orders

(Please make sure to update this or tell Meagan if you receive an order -- and make sure to save the packing slip) [show][hide]

100 cylindrical 0.125x0.125 magnets
100 cube 0.125 x 0.125 magnets
50 cube 0.187x0.187 magnets
ReqID: 11104249
- case N-DEX gloves, large
- case N-DEX gloves, medium
- cases of 10,20,200,and 1000 pippette tips (no full cases of any left, though there are a few boxes of the 1000s)
Invitrogen PCR SuperMix
Invitrogen ChargeSwitch CS10201 (for assembly)
Roche Protease Inhibitor, Complete Mini, EDTA-Free, 11836170001
Sigma D-7384-100G, Decanal
NEB EcoRI-HF R3101S
2x boxes Invitrogen 12-well 0.8% E-Gels
Req ID: 11084279
- 2 case petri dishes
- GL45 media bottle caps, membrane, blue, Kimax 14395M45, VWR 89000-948, box 10
Qiagen miniprep kit --Meaganl 11:45, 21 November 2008 (EST)
NEB EcoRI R101S --Meaganl 11:45, 21 November 2008 (EST)
NEB T4 DNA ligase M0202S --Meaganl 11:45, 21 November 2008 (EST)
3x Invitrogen NuPage sample reducing agent (10x) 250 ul, cat NP0004
L-Arabinose- A3256-100G Sigma
3x Difco LB Agar, Lennox, 500g
2x gal bleach
Difco Agar Noble 500g Ref 214230 (ON BACKORDER)
MRS broth, BD 288130, 500g VWR 90004-082 (Temporarily on backorder)
Case Microcentrifuge Tube Boxes, 1.5 ml, Nalge/Nunc 5055-5015, VWR 55710-175
NUNC 5-spot grey holder boxes VWR #534479
20x Gibco yeast extract Cat. # 18180-059
Sigma C5080-500g Calcium chloride dihydrate, Sigma-Ultra
large latex gloves evolution one Reorder #: EV-2050-L
Case Kimwipes, EX-L
Sterile reagent reservoirs 100ml case 100 VWR 82026-358
3x pack 300 disposable spatula, standard, opaque, VWR 80081-190
Beckman Futura pH electrode, 511083 Calomel, 5mm x 225 mm VWR BK511083
Sigma M1633-1G 4-Methylumbelliferyl beta-D-galactopyranoside
6x Sigma H1138 horse serum, donor herd, heat inactivated, 500 ml
2x EMD OmniPur Sucrose 500g
3mm glass balls (for spreading transformed cells on plates)- no more left
Difco Heart Infusion Broth Ref. # 238400, 500 g
1 case 10 Axygen MCT-200-C-S microtubes
1 case 10 Axygen MCT-175-C-S microtubes

(Req ID: 11027695)

2x Zymo Genomic DNA prep plates 4x 96 well plate kit (D3011)
nalgene cryoware cryogenic vials Cat #5000-0020 (last case, 2 bags left-order Thurs. July 10th if possible)
case BD 305491 5 gal sharps containers (VWR BD305491)
2x pack 50 VWR electroporation cuvettes, 1 mm, VWR 89047-206
3x VWR aluminum foil plate seals, sterile, pack 50, VWR 60941-076
14 mL culture tubes
case 1000 BD 352054 5ml culture tubes (VWR 60819-310)
case 72 VWR Traceclean 20 ml vials with fluoropolymer resin/silicone septum VWR 15900-008
case 72 Nalgene boston round bottles, 8 ml, polypropylene, NNI 2006-9025, VWR 16066-981
2 cases 200 ul pipet tips, barrier, sterile, racked VWR 14217-728
NEB M0201S T4 polynucleotide kinase
2 x TOP10 one-shot chemically competent cells SKU# C4040-03
Egel 12 lane gels .8 or 1%, only 5 or 6 are left
Qiagen RNeasy Protect Bacteria Mini Kit Cat. #74524
6x Sigma H1138 horse serum, donor herd, heat inactivated, 500 ml
Qiagen RNase-Free DNase Set Cat. #79254
ReqID: 11013874 (for PO)
- 10x Rainin 250ul multichannel tips (SR-L250S)
- 10x 1200ul multichannel tips
2x DpnI from NEB (amount in -20 freezer restriction enzyme box is gone) Note: You can get the following free from NEB

Receive a free Protein Ladder Sample with any purchase.

2x NEB Phusion Flash high fidelity master mix F-548L (large version)
EMD OmniPur Sucrose 500g
3M Micropore breathable cover for 96-well plates
4x Zymo Genomic DNA prep plates 4x 96 well plate kit (D3011)
2x Invitrogen S.O.C. medium 10x 1 ml, #15544-034
Costar 3370 (96 well flat bottom assay plate)
3x VWR aluminum foil plate seals, sterile, pack 50, VWR 60941-076
2x Rainin 250ul multichannel tips (SR-L250S)
2x 1200ul multichannel tips
NEB BfuCI R0636S
Sigma beta-galactosidase G4155-1KU
large latex gloves evolution one Reorder #: EV-2050-L
greiner 96-well plates catalog #T-3026-16
3x VWR aluminum foil plate seals, sterile, pack 50, VWR 60941-076 (ReqID: 11000465)
Invitrogen PCR SuperMix (only 2 tubes left) (ReqID: 11000464)
Rainin 250ul multichannel tips (SR-L250S)
1200ul multichannel tips
ReqID: 10996069
- Case 50, Greiner 2 ml clear Masterblock deep well plates, sterile, Greiner 780271, VWR 82051-248
- 2x case 50, Nunc Microwell 96-well polystyrene plates, sterile, 0.3 ml, V-bottom, Nunc 249662, VWR 62409-112 (ON BACKORDER - SHIPPING 5/12)
- 2x case 50, Nunc Microwell plate lids, sterile, cut off corners, Nunc 264122, VWR 62409-118 (ON BACKORDER - SHIPPING 5/6)
- 2x case 500, Axygen self-standing conical screw cap tubes, sterile, assorted colors, Axygen SCT-050-SS-A-S, VWR 10011-502 (ON BACKORDER - SHIPPING 5/22)
2x NEB Sau3AI R0169S (ON BACKORDER INDEFINITELY - DO YOU WANT TO CANCEL?)
Sciencelab 60-136380518 polyethylene fritware sheet, 70 micron, 18"x18" 1/8" thick
- http://sciencelab.com/page/S/PVAR/60-136380018
ReqID: 10913140
- Daigger FX23461AX 96 well PCR racks, assorted colors, 4x pack 5 (on backorder --Meaganl 11:46, 27 August 2007 (EDT))
Get these Greiner plates instead: Endy:Victor3_plate_reader/Plates (catalog # T-3026-16) (reqID: 10996115)
20x Invitrogen/Gibco yeast extract 18180-059 (ReqID# 10996112)
6x Zymo Genomic DNA prep plates 4x 96 well plate kit (D3011) (Order # 107945)
2x NEB Buffer 2 pack, B7002
Rainin 10ul multichannel tips (SR-L10S)
ReqID: 10996069
- 3x Sterile reagent reservoirs 100ml case 100 VWR 82026-358
- 3x Petri-stickers, Diversified Biotech PSTK-1050, [1]
- 3x cases 200 ul pipet tips
- 2x cases 20 ul pipet tips
- 2x pack 50 VWR electroporation cuvettes, 1 mm, 89047-206
- Difco Heart Infusion Broth, 238400, 500g
- Difco/BD 220215 sterile disposable inoculating loop, 1 ul, 50/tube, pack 1000, VWR 90001-098
ReqID:
- 6x Sigma H1138 horse serum, donor herd, heat inactivated, 500 ml
- Sigma I1284 IPTG
NEB protein ladder, P7703S
Rainin 250ul multichannel tips
2x NEB DpnI R0176S
aluminum foil
2x gal Pharmco ethanol, 190 proof
2x gal Pharmco ethanol, 200 proof
10x Invitrogen/Gibco Yeast extract solution, 100 ml, cat. 18180-059
pack 50 VWR electroporation cuvettes, 1 mm gap VWR 47727-640
pack 50 VWR electroporation cuvettes, 2 mm gap VWR 47727-642
3x cases petri dishes
Axygen screw top microtubes, amber, 0.5 ml, self standing, ST-050-SS-X, VWR 10011-656, pack 500
2x case 10 ml pipets
2x case 25 ml pipets
2x case 50 ml pipets
Pfu turbo DNA polymerase catalog no 600250 from Stratagene
Qiagen ERC MinElute cleanup kit large #28206
20x Brother TZ-241 3/4" black on white label tape
Sigma M1633-250MG 4-Methylumbelliferyl beta-D-galactopyranoside
NEB PvuII R0151S
Sigma 43816-50ML, DTT, 1 M solution, 50 ML
Sigma D0632-10G, DTT, 10g
Invitrogen NuPage MOPS SDS running buffer, 20X, 500 ml, cat NP0001
Invitrogen NuPage LDS sample loading buffer (4x) 10 ml, cat NP0007
Invitrogen NuPage sample reducing agent (10x) 250 ul, cat NP0004
NEB Chitin beads S6651L (large version)
pack 50 VWR Signature electroporation cuvettes, 2mm gap, VWR 89047-208
sterile inoculating loops
NEB B0202S T4 DNA Ligase buffer pack (4x 1.5 ml buffer)
2x Invitrogen NuPage 10% Bis-Tris Gels, 1.0mm x 9 well, cat NP0307BOX
ReqID: 10970539 (6)
- 2x Difco SOB medium 244310, 500g (On backorder)
- 4x gal bleach
Fermentas PpiI, cat #: ER1541, 50 units, $66
SibEnzyme MabI, cat #: E121, 200 units, $40
SibEnzyme PsrI, cat #: E131, 100 units, $50

(-Julie)

ReqID: 10975136
- Molec bio grade ethanol (Sigma 7023-500ml)
ReqID: 10975027
- (3) NUNC Universal lids, sterile (VWR 73520-150, Nunc 250002)
ReqID: 10974958
- Qiaprep Spin Miniprep Kit (250), Cat. #27106
- Reagent reservoirs
- (5) Teknova Amp plates
ReqID: 10974975
- GE(Amersham) templiphi 100
Epicentre EZ-Tn5 transposase
2x case 12 Nalgene SFCA bottle top filters, 500 ml, VWR 291-4520
10ul tips (VWR 53509-500)
NEB EcoRI
NEB XbaI
NEB T4 PNK M0201S
ReqID: 10968275
Sterile inoculating loops (VWR 12000-810)
Teknova AMP plates (VWR 100216-550)
Media cart for holodeck
Corning 96-deepwell plates (3960) - 6 cs
ReqID: 10967075
- Glycerol
- Sigma C5080 Calcium Chloride dihydrate, 500g
ReqID: 10967081
- Microgrip Nitrile Gloves (40101-346)
- half-height round bottom 96-well plates - 3cs (82051-244)
ReqID: 10963590
- Pierce 31503 Superfreeze peroxidase conjugate stabilizer, 25 ml
Costar 3960 (96 deep well culture plates)
1 case 10 Axygen MCT-200-C-S microtubes
ReqID: 10963547
- 0.8% 12 well e-gels from Invitrogen (G5018-08)
- Invitrogen 10x TAE
ReqID: 10963590
- 3x case 200 ul pipet tips (VWR 14217-728)
- Falcon 14ml tubes 352059 (VWR 60819-761)
- freezer boxes (w foam inserts) (Nalgene 5055-5015)
ReqID: 10963609
- PBS (pH 7.4) 1L
EZ-Tn5 transposase
1 case 10 Axygen MCT-060-C-S microtubes
1 case 10 Axygen MCT-175-C-S microtubes
3x case Nalgene Cryoware Cryo Vials, Cat # 5000-0020
2x case 10 ml disposable pipets
2x case 50 ml disposable pipets
ReqID: 10956768
- Reagent reservoirs, VWR 82026-358
- Teknova Amp plates (L1004)
- 4 bottles LB agar (240110)
- Spiral bound computation books (lab notebooks). Ampad #22-157. VWR TX126643MT
- Autoclave bags, 25" x 35", VWR 14220-042
- Petri dishes, two cases (25384-250)
ReqID: 10954559
- Invitrogen PCR Supermix
10 ul tips
--Meaganl 13:43, 9 January 2008 (CST)
- Req ID: 10949471
  - 2x Sucrose, EMD Omnipure, 500 g
- 20x Brother TZ-241 3/4" black on white label tape
- Req ID: 10949254
  - Difco 0142 Agar Noble, 500g
  - 2 dozen Campingaz / Coleman CV360 butane canisters (for Fireboy) VWR 17919-890
  - 1000ul tips
- Pierce EZ-link Psoralen-PEO-biotin, 5 mg #29986
- Pierce Streptavidin Horseradish peroxidase conjugated, 1 mg, # 21126 (in -20)
- Pierce Immunopure biotinylated horseradish peroxidase, 5 mg, # 29139
- Pierce One-step Ultra TMB-substrate, 250 ml # 34028
- Pierce Ultralink immobilized streptavidin, 2 ml, # 53113
- Req ID: 10949274
  - Sigma M0262 Methylcellulose viscosity 400 cP, 2 % in H2O, 100g
  - Sigma E5529 EZ-view red streptavidin affinity gel, 1 ml
- case BD sharps collector 3.1 liter BD 305488
- Qiagen ERC MinElute cleanup kit large #28206
Req ID: 10937895
- Nitric acid, concentrated, 1 liter
NEB BsmBI R0580S
ReqID: 10946402
- Invitrogen Sybr Safe
ReqID: 10946457
- Sigma Aldrich 151238 Glycidyl methacrylate 5 grams
- Sigma B9300 Benzophenone 500 grams
- Sigma Aldrich 311448 Sodium periodate 5 grams
- Sigma S4762 Streptavidin 1 milligram
- Sigma T4174 10×, 5.0 g porcine trypsin and 2 g EDTA • 4Na per liter of 0.9% sodium chloride 100 ml
- Sigma T4799 Trypsin from procine pancreas 5 grams
- Sigma T9003 Type I-S Trypsin inhibitor, lyophilized powder (Sigma), 100 milligrams
200ul tips
ReqID: 10943944
- 5x horse serum, Sigma
10x Invitrogen/Gibco Yeast extract solution, 100 ml, cat. 18180-059
ReqID: 10943885
- Qiaprep Spin Miniprep Kit (250), Cat. #27106
8 strip ultra clear caps Cat No TCS-0803 clear from MJ Research (now BioRad - same cat. number)
Low tube strip, CLR from BioRad catalog no. TLS0801 (please buy equal numbers as caps
Rainin SR-L250S multichannel tips (250 uL capacity)
Harris Uni-Core punches, 0.35, 0.50, 0.75, 1.0 mm, four each, Ted Pella
Hydrochloric acid, concentrated, 1 liter
EZ-Tn5 transposase, Epicentre, EZ-Tn5
Req ID: 10937925
- 6x Horse Serum, Donor Herd, Sigma H1138, 500 ml
200μL multichannel tips from Rainin
Acetic acid, glacial, 1 liter
ReqID: 10936396
- 2x case 5 VWR 87006-060 Barrier tips, sterile, extended length, 1000 ul
- large latex gloves evolution one Reorder #: EV-2050-L
- Difco LB Agar, Lennox, 500g
1 box Invitrogen PCR Supermix High Fidelity cat. 10790-020
multichannel 10ul tips Rainin SR-L10F
Ptouch label supplies 3/4in white background, black ink - TZ-241
Zymo ZR96 genomic DNA kit, 4 x 96 well plates D3011, Zymo site
Amylose resin from NEB (Catalog no. E8021S) --Reshma 14:38, 25 November 2007 (CST)
NEB PstI
P1000 tips
Qiaprep Spin Miniprep Kit (250), Cat. #27106 (only 10 or so columns left may want to order before Thurs)
Qiagen Qiaex II Gel Extraction Kit, Cat. #20051
NEB MboI (large) R0147L
1 NEB T4 DNA Ligase M0202S (need before Thursday)
Invitrogen Sybr Safe
ReqID: 10930951
- Case Microcentrifuge Tube Boxes, 1.5 ml, Nalge/Nunc 5055-5015, VWR 55710-175
- 2x case 12 Nalgene 291-4520 SFCA Bottle Top Filter 75mm dia filter, 45 mm cap, VWR 28199-307
- 3x Novagen Pellet Paint NF #70748 (available through VWR)
- 2x case Nalgene cryo vials Nalgene 5000-0020
Sau3AI, NEB R0169S
PvuII, NEB R0151S
Phusion HF master mix, NEB F531S
pack 50 VWR Signature electroporation cuvettes, 2mm gap, VWR 89047-208
ReqID: 10923808
- 2-Nitrophenol (Sigma Aldrich N19702-5G)
EZ-Tn5 transposase, Epicentre, EZ-Tn5
Req ID: 10920911
- VWR 12x75mm polypropylene round bottom sterile tubes Cat No 60818-576
- BD Falcon 352054 5ml polystyrene falcon tube
- 2x cases 50 ml plug seal centrifuge tubes, sterile, racked (VWR 20171-028 --Meaganl 12:31, 17 October 2007 (CDT))
- 2x cases 15 ml plug seal centrifuge tubes, sterile, racked (VWR 20171-024 --Meaganl 12:31, 17 October 2007 (CDT))
- 2x 1/4 oz. Immersion oil type DF low fluorescence, CARGILLE LABORATORIES, VWR 48218-506
VWR 33725-042 Glas-Col Mini-rotator (099A-MR1512) 120v (another one, now that we know they work well)
2x boxes 12 well 0.8% agarose E-Gels
exo I M0293S
DpnI R0176S
ReqID: 10915844
- Sodium deoxycholate from Sigma 30970-25G
MboI NEB R0147S
2x cases Petri dishes
Pack 768 VWR 87006-060 Barrier tips, sterile, extended length, 1000 ul (on backorder)
Daigger FX4727A 250 ml polypropylene centrifuge tubes, 250 ml, Pack 12 (on backorder --Meaganl 11:46, 27 August 2007 (EDT))
VWR 33725-042 Glas-Col Mini-rotator (099A-MR1512) 120v (On backorder until 9/24) --Meaganl 14:45, 20 September 2007 (EDT)
ReqID: 10906942
- Falcon 14ml tubes 352059 (3 boxes, we go through them fast)
- 3x Drummond Portable Pipet-Aid rechargeable battery Drummond 4-000-035 VWR 53498-063
- case 12, Nalgene MF75 Bottle top vacuum filters, Surfactant free CA, 500 ml, 0.20 micron, NNI 291-4520, VWR 28199-307
- ART 10ul tips
- Pack 768 VWR 87006-058 Barrier tips, sterile, 250 ul
3 boxes Invitrogen PCR Supermix High Fidelity cat. 10790-020
6x Invitrogen/Gibco Yeast extract, 100 ml, cat. 18180-059
ReqID: 10904172
- Roche Dig-High Prime DNA labeling and detection starter kit II for chemiluminescent detection cat 11 585 614 910
- Roche Digoxigenin-11-dUTP, alkaline labile 25 nmol, cat 11 573 152 910
ReqID: 10904145
- BD 35 2059 polypropylene round bottom tubes, 14 ml, 17 x 100 mm
ReqID: 10904171
- Sigma 77617 Fluka Biochimika ultra Phenol Chloroform Isoamyl alcohol mixture 25:24:1, 100 ml
ReqID:10898629
Pierce B-PER bacterial protein extraction reagent 500 ml #78248
Req ID: 10901718
- K3007 N-(β-Ketocaproyl)-L-homoserine lactone from Sigma
Invitrogen UltraPure Agarose 15510-027
Invitrogen Sybr Safe
ReqID: 10901688
- 3x pack 1000 MCT-060-C-S
- 1x case 10 MCT-175-C-S
- 1x case 10 MCT-200-C-S
- 2x pack 100 Axygen Cyclerseal PCR-TS, VWR 10011-120
- case 36 BD 353226 24 well plates, 6/bag, VWR 62406-159
NEB P8101S Modified Trypsin
NEB S6651S Chitin beads
NEB M0280S UDG
Roche Complete mini EDTA free protease inhibitor cocktail, glass vial 25 tablets, 11 836 170001 --Meaganl 10:12, 4 September 2007 (EDT)
3x Crane's Thesis Paper100% Cotton, Acid Free, 500 sheets (SKU BT886S)
Daigger FX28271J 6x10 4 mil polyethylene bags, case 1000
recombinant EGFP 100ug (Order # 120924)
Nalgene Cryoware Cryo Vials, Cat # 5000-0020 --Meaganl 10:50, 22 August 2007 (EDT)
2x VWR 55411-050 single edge razor blades, pack 100 --Meaganl 10:50, 22 August 2007 (EDT)
Qiagen miniprep kit
Sap1 restriction enzyme
95% ethanol
100x Olfa Green Mat, 6" x 8
6x Invitrogen 18180-059 Yeast extract solution --Meaganl 14:05, 14 August 2007 (EDT)
2x Invitrogen E-Gel .8% Agarose, Cat #G5018-08 --Meaganl 14:05, 14 August 2007 (EDT)
Pfu turbo DNA polymerase catalog no 600250 (100 U) from Stratagene.--Meaganl 14:05, 14 August 2007 (EDT)
100x Harris Uni-core punch, disposible, 2mm
Req ID: 10893241
- 2x pack 50 VWR Signature electroporation cuvettes, 1mm gap, VWR 47727-640 --Meaganl 11:49, 14 August 2007 (EDT)
Topoisomerase 1 from E. coli 100 units catalog # M0301S
Epicentre EZ-Tn5 transposase TNP92110, 10 units
Req ID: 10890897
- 3x cases 20 ul tips
- 3x cases 200 ul tips
- 3x cases 1000 ul tips
Invitrogen Sybr Safe
Harris Uni-core punch, disposible, 2mm
- http://www.tedpella.com/histo_html/unicore.htm
C. S. Osborne Belt punches, sizes 00, 0, 1, 2
- http://www.siegelofca.com/itemdetail.asp?prodid=411
Olfa Green Mat, 6" x 8"
- http://olfablades.stores.yahoo.net/rm6x8.html
Ptouch 3/4" white black ink labelling supplies TZ-241 (10x)
Qiagen Minelute PCR Cleanup Kit (250) # 28006
2-log Ladder (from New England Biolabs) #N3200L
Invitrogen E-gel-48
NuPAGE® Novex 4-12% Bis-Tris Gel 1.0 mm, 12 well NP0322BOX - 1 box
Req ID 10887733
- Spiral bound computation books (lab notebooks). Ampad #22-157. VWR TX126643MT
- 100ml reservoirs VWR 82026-358
- Falcon 14ml tubes 352059
- Nalgene blue 16mm test tube racks (for holding 14ml tubes)- 60914-730
Req ID: 10886117
- Difco LB Agar Lennox Ref. 240110
Foil adhesive covers (60941-074)
3 boxes Invitrogen PCR Supermix High Fidelity cat. 10790-020
ReqID: 10883548
- 2x cases petri dishes
- 2x cases 12 Nalgene 290-4520 SFCA bottle top filter, 150ml, 45mm neck, 50 mm membrane, 0.2u filter
- 2x PstI R0140S
- Robot: SpeI
ReqID: 10878908
- Bleach
multichannel 10ul tips Rainin SR-L10F
VF2 and VR Primers
Robot: 30ml and 100ml reservoirs
Alconox Alcojet dishwashing detergent (let me know if you need a catalog no.)
ReqID: 10874005
- Sigma C7901-25G Chelex 100
SeeBlue® Plus2 Pre-Stained Standard - Invitrogen Catalog Number - LC5925
Robot: Invitrogen E-gel 48
MicroAmp™ Optical 96-Well Reaction Plate (Part# N8010560) - 10 Plate Package
2x E-Gel 0.8% agarose (GP) #G5018-08
ReqID: 10871228
- TempliPhi 100 Amplification Kit
NEB phi29 DNA polymerase M0269S
Beckman Futura pH electrode, 511083 Calomel, 5mm x 225 mm VWR BK511083
T4 endo VII http://www.mclab.com/product.php?productid=18100&cat=292&page=1
TDG H00006996-Q01 http://www.novusbio.com/data_sheet/index/H00006996-Q01
NEB BbsI R0539S
Robot: Sigma ATP (3x)
1 500 mL bottle of Ethyl acetate catalog # MK499204 at VWR
- BD Falcon 352063 (VWR 60819-728)
- 14 mL culture tubes Falcon 352059 (VWR 60819-761)
DNA Topoisomerase I, Vaccinia (500 U)
Propidium iodide Invitrogen P1304MP
NuPAGE® Sample Reducing Agent (10X) NP0004
NuPAGE® Novex 4-12% Bis-Tris Gel 1.0 mm, 12 well NP0322BOX
Immobilized Streptavidin Pierce #20349 (VWR # PI20349)
100 ml multi-well pipettor reservoirs
Spherotech ACFP-100-3 AccuCount Fluorescent Particles 8.0-12.9 µm, 3 mL
3x Novagen Pellet Paint NF #70748 (available through VWR)
Qiagen miniprep spin kit (250) # 27106
Qiagen Minelute PCR Cleanup Kit (250) # 28006
NEB M0302S endo I
NEB M0305S endo V
(2cs) Petri dishes
Sigma M2128 Thiazolyl Blue Tetrazolium 500 mg
K3007 N-(β-Ketocaproyl)-L-homoserine lactone from Sigma
polypropylene tubes VWR catalog# 60818-576
Spherotech RCP-60-5 calibration beads
8 well strip tubes, clear, low profile
1 case of 10ul tips (not via requisition; on CC)
Req ID: 10855012 Ampicillin salt (Sigma A9518)
Req ID: 10854533 TempliPhi 100 (Amersham/GE Healthcare)
3 boxes Invitrogen PCR Supermix High Fidelity cat. 10790-020
Req ID: 10854540
- two dozen black ultra-fine point Sharpies
Req ID: 10854530
- case BD 305491 5 gallon sharps containers
- case Nalgene 5055-5015 microcentrifuge box, 64 places, foam insert
- 2 cs 10ul tips
- 6 cs 20ul tips
- 3 cs 200ul tips
- 3 cs 1000ul tips
scotch tape. The rolls that can be put in existing dispensers.
large latex gloves
Mermaid kit, 200 preps, [2]
Geneclean III kit, 600 preps, [3]
(3) NEB T4 DNA Ligase M0202S
NEB phi29 DNA polymerase M0269S
ReqID 10851873
- Sigma D157805-5G N,N'-dimethylethylenediamine
Invitrogen Sybr Safe
PO# 10847064
- Nonidet-P40 substitute Sigma 74385-1L
- Tween-20 Sigma P7949-100ML
- Tween-40 Sigma P1504-100ML
- Tween-80 Sigma P8074-100ML
Glycerol (Sigma G6279) -- on backorder
1.7mL clear sterile tubes (Reshma) --Meaganl 09:43, 12 April 2007 (EDT)
Reshma 09:41, 19 March 2007 (EDT): Cartridges for Ptouch labeller. 18mm, 3/4", Black ink on white, TZ-241 TZ tape
Trevigen TDG 4070-500-EB
Trevigen MutY 4000-500-K
Beckman Futura pH electrode, 511083 Calomel, 5mm x 225 mm VWR BK511083
Beckman reference solution 4M KCl, 4 x 100 ml, 566468 VWR BK566068
--Meaganl 14:44, 3 April 2007 (EDT)
- Reshma 11:55, 23 March 2007 (EDT): SpeI
- NEB EarI (yes again) R0528S
- NEB endo IV M0304S
- NEB endo VIII M0299S
Qiagen miniprep kit (large)--Meaganl 10:32, 3 April 2007 (EDT)
Immobilized Streptavidin Pierce #20349 (VWR # PI20349) --Meaganl 13:42, 26 March 2007 (EDT)
Reshma 11:23, 19 March 2007 (EDT): Nalgene cryovials
Reshma 17:06, 8 March 2007 (EST): 14 mL culture tubes Falcon 352059
Reshma 13:44, 8 March 2007 (EST): Difco LB Lennox Broth
NEB BsmBI R0580S --Meaganl 10:41, 22 March 2007 (EDT)
Ambion superase-in #2694 --Meaganl 15:16, 15 March 2007 (EDT)
Reshma: Qiagen ERC MinElute cleanup kit large #28206
Reshma: PCR supermix
--Meaganl 11:05, 9 March 2007 (EST)
- case BD sharps collector 3.1 liter 305488
- case standard petri dishes 100 x 15 mm VWR # 25384-250
- 3x bottles bleach
- 4 cases 1000 ul barrier pipet tips VWR 14217-730
- 4 cases 200 ul barrier pipet tips VWR 14217-728
- 3 cases 50 ml serological pipets BD 357550
- 3 cases 10 ml serological pipets BD 357551
- 1 case 25 ml serological pipets BD 357525
NEB phi29 DNA polymerase M0269S --Meaganl 16:29, 8 March 2007 (EST)
NEB BbsI R0539S --Meaganl 16:29, 8 March 2007 (EST)
NEB EarI R0528S --Meaganl 16:29, 8 March 2007 (EST)
Invitrogen Sybr Safe --Meaganl 16:27, 6 March 2007 (EST)
NEB T4 DNA ligase large (M0202L) PO# 0010830023 --Meaganl 08:49, 2 March 2007 (EST)
VWR 3 1/2 mm glass beads 1lb pack 26396-521 (PO# 10826298) tk 15:32, 26 February 2007 (EST)
Invitrogen E-Gels, 0.8% EtBr, Single comb, pack 18, G5018-08 tk 15:32, 26 February 2007 (EST)
PO #10825054
- Sigma Aldrich Tetracycline BioChemika 87128-25G tk 15:32, 26 February 2007 (EST)
- Sigma Aldrich Chloramphenicol BioChemika 23275-25G tk 15:32, 26 February 2007 (EST)
- Sigma Aldrich Kanamycin K1637-5G tk 15:32, 26 February 2007 (EST)
PO# 10823861
- GE Healthcare Sephacryl S-500 HR 150 ml, Cat 17-0613-01 VWR 95016-920 tk 15:32, 26 February 2007 (EST)
- GE Healthcare Sephacryl S-400 HR 150 ml, Cat 17-0609-01 VWR 95016-912 tk 15:32, 26 February 2007 (EST)
- GE Healthcare Sephacryl S-300 HR 150 ml, Cat 17-0599-01 VWR 95016-908 tk 15:32, 26 February 2007 (EST)
Polybead polystyrene red dyed microspheres, 0.2 micron, 2.5% aqueous solution, smallest amount tk 15:32, 26 February 2007 (EST)
- Order # 47T9282PPGGQ8LDHCRF9WDNN82
PO#10822102
- Sucrose, Omnipure, EMD, VWR #EM-8510, 500g
PO#10822065
- 3x pack 300 disposable spatula, standard, opaque, VWR 80081-190
- NEB BsmBI
- 2x NEB 2-log ladder, large N3200L
P-10 tips (2 cases) --Meaganl 09:44, 2 February 2007 (EST)
Multichannel 1200ul pipette tips --Meaganl 09:44, 2 February 2007 (EST)
Bio-rad PCR strip tubes #TLS0801 (5 boxes) tk 19:13, 31 January 2007 (EST)
NEB β Agarase I M0392S --Meaganl 14:37, 31 January 2007 (EST)
Epicentre CircLigase #CL4111K --Meaganl 13:36, 30 January 2007 (EST)
Micropure EZ columns Catalogue Number: 42530 from Millipore (shipped on 1/26)--Meaganl 13:36, 30 January 2007 (EST)
Shipped on 1.26 --Meaganl 14:22, 29 January 2007 (EST)
- Qiagen RNase-free Dnase set Cat #79254
- Qiagen RNeasy minelute cleanup Cat #74204
- Qiagen ERC MinElute cleanup kit #28204
EDTA disodium salt for molecular biology 500g Sigma E5134 --Meaganl 09:48, 24 January 2007 (EST)
Sigma S7547-1Kg D-Sorbitol Sigmaultra --Meaganl 09:48, 24 January 2007 (EST)
Sigma M6880-25G (malachite green oxalate salt) --Meaganl 09:48, 24 January 2007 (EST)
DEPC - 25mL (Sigma) --Meaganl 09:48, 24 January 2007 (EST)
2x packs 100, BD Syringe 3 ml Luer Lok, BD 309585 --Meaganl 09:48, 24 January 2007 (EST)
NEB N0345S Yeast Chromosome PFG Marker --Meaganl 21:36, 23 January 2007 (EST)
NEB N0340S Lambda ladder PFG Marker --Meaganl 21:36, 23 January 2007 (EST)
PMSF 100 mM in ethanol 50 ml Biochemika 93482 --Meaganl 14:56, 22 January 2007 (EST)
N-lauroyl sarcosine sodium salt 30% solution 100 ml Biochemika 61747 --Meaganl 14:56, 22 January 2007 (EST)
2 cases standard petri dishes 100 x 15 mm VWR # 25384-250 --Meaganl 14:56, 22 January 2007 (EST)
4L cubetainer of 10x TBE buffer VWR EM-8820 --Meaganl 14:56, 22 January 2007 (EST)
ClaI NEB R0197S --Meaganl 14:56, 22 January 2007 (EST)
P-10 tips --Meaganl 14:56, 22 January 2007 (EST)
2 4L cubetainers of 10X TAE buffer Invitrogen #15558-026 --Meaganl 11:48, 18 January 2007 (EST)
SYBR gold (Invitrogen catalog number S-11494) --Meaganl 11:48, 18 January 2007 (EST)
Invitrogen Sybr Safe --Meaganl 11:48, 18 January 2007 (EST)
Nunc Deepwell 384-well plates
EcoR1, small (Robot)
SpeI, large (Robot) x2
PstI, small (Robot)
Eppendorf Robot Miniprep kits x2 --Reshma 15:17, 10 January 2007 (EST)
XbaI --Reshma 15:17, 10 January 2007 (EST)
Difco LB Agar, Lennox--Meaganl 10:39, 9 January 2007 (EST)
illustra TempliPhi 100 Amplification Kit (Amersham/GE Healthcare) tk 22:01, 22 December 2006 (EST)
200 ul pipet tips (3 cases)--Meaganl 07:46, 21 December 2006 (EST)
20 ul pipet tips (2 cases)--Meaganl 07:46, 21 December 2006 (EST)
1000 ul pipet tips (3 cases)--Meaganl 07:46, 21 December 2006 (EST)
Falcon 14ml tubes 352059--Meaganl 07:46, 21 December 2006 (EST)
AG 501-X8(D) Resin, molecular biology grade, 100 g, catalog number 143-6425 from Bio-Rad--Meaganl 07:46, 21 December 2006 (EST)
Sigma Molecular Bio Grade 200 proof ethanol--Meaganl 10:12, 20 December 2006 (EST)
RNaseZap wipes (Ambion AM9786)--Meaganl 10:12, 20 December 2006 (EST)
Alpha Innotech (replacement) EtBr filter EBR-500K
Qiagen miniprep kit (2 of these I believe) --Reshma 13:52, 9 December 2006 (EST)
Eppendorf 30ml robot reagent reservoirs --Meaganl 14:22, 3 December 2006 (EST)
100ml sterile reagent reservoirs--Meaganl 11:26, 1 December 2006 (EST)
(VWR) Nalgene microcentrifuge tube boxes (case)--Meaganl 11:26, 1 December 2006 (EST)
Plastic disposible cuvettes, OPS, 10x10x45 mm, 2 optical surfaces, VWR 58017-825, case 500--Meaganl 11:26, 1 December 2006 (EST)
Plastic disposible cuvettes, OPS, 10x4x45 mm, 2 optical surfaces, VWR 58017-847, case 500--Meaganl 11:26, 1 December 2006 (EST)
Difco Bacto Agar 0140-01--Meaganl 11:26, 1 December 2006 (EST)
Sigma L5263-25KU, Lyticase from Arthrobacter luteus, partially purified--Meaganl 15:33, 30 November 2006 (EST)
Sigma 73660-5G Biochemika 99%+ 2-nitrophenyl β-D-galactopyranoside (ONPG)--Meaganl 15:44, 29 November 2006 (EST)
SpeI NEB R0133S--Meaganl 15:04, 28 November 2006 (EST)
NotI NEB R0189S--Meaganl 15:04, 28 November 2006 (EST)
β-Agarase I NEB M0392S--Meaganl 15:04, 28 November 2006 (EST)
Sigma 43816-50ml DL Dithiothreitol solution 1M Biochemika ultra -- tk 14:18, 27 November 2006 (EST)
Sigma S7547-1Kg D-Sorbitol Sigmaultra --Meaganl 11:30, 27 November 2006 (EST)
Sigma D5545-1G DL Dithiothreitol Sigmaultra --Meaganl 11:30, 27 November 2006 (EST)
Brother Labeler 18mm 3/4" black ink on white tape --Meaganl 10:06, 27 November 2006 (EST)
Nalgene microcentrifuge tube boxes (only got 4 boxes) --Meaganl 11:22, 20 November 2006 (EST)
BD 60ml syringes --Meaganl 11:22, 20 November 2006 (EST)

@@ Line 6: / Line 6: @@
 ==Needs to be Ordered==
-* adhesive foil covers (VWR 60941-076)
-* LB broth
 * 96-deepwell plates (for DeLong MP robot)
 * Teknova plates
 * petri plate labels (for QC)
 *[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx Acinetobacter baylyi ATCC 33305]
-* Fireboy gas canisters (VWR 17919-890)
-* NEB streptavidin magnetic beads S1420S
 ==Placed Orders==
+* NEB streptavidin magnetic beads S1420S
+*ReqID 11106769
+** Fireboy gas canisters (VWR 17919-890)
+** adhesive foil covers (VWR 60941-076)
+** LB broth
 *ReqID: 11105446
 ** Invitrogen PCR Supermix High Fidelity (Invitrogen 10790-020)

Knight:Orders: Difference between revisions

Revision as of 08:36, 22 January 2009

Needs to be Ordered

Placed Orders

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