Austin J. Che: New page: See beta-galactosidase assay for other methods. This method uses 4-Methylumbelliferyl beta-D-galactopyranoside (MUG) for lacZ detection (Sigma M1633). MUG has the advantage that it ge...

Austin J. Che — Fri, 04 Apr 2008 22:15:44 GMT

New page: See beta-galactosidase assay for other methods. This method uses 4-Methylumbelliferyl beta-D-galactopyranoside (MUG) for lacZ detection (Sigma M1633). MUG has the advantage that it ge...

New page

See [[beta-galactosidase assay]] for other methods.

This method uses 4-Methylumbelliferyl beta-D-galactopyranoside (MUG) for lacZ detection (Sigma M1633). MUG has the advantage that it gets into the cell. MUG can be detected via fluorescence using 360nm/460nm

==Materials==
* 2 mg/ml MUG in DMSO. Use as 10x (final concentration 200 μg/ml)

== Notes ==
* It works to grow liquid cultures with MUG. The fluorescence is easily detected on a normal UV box (cells look blue)
* The MUG can be directly substituted for ONPG in a [[Beta-Galactosidase_Assay_(A_better_Miller)|Miller-type]] assay
* It is highly amenable to reading on a plate reader in 96-well format

<biblio>
#Vidal-Aroca-2006 pmid=16629389
</biblio>

[[Category:Protocol]]
[[Category:Escherichia coli]]

Knight:Beta-galactosidase assay/Fluorescence - Revision history

Austin J. Che: New page: See beta-galactosidase assay for other methods. This method uses 4-Methylumbelliferyl beta-D-galactopyranoside (MUG) for lacZ detection (Sigma M1633). MUG has the advantage that it ge...