Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/29: Difference between revisions
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=29 April 2010 Lab Meeting= | |||
* Senior Project Practice presentations. <br> | |||
'''‡ Announcements'''<br> | |||
* May 6 Lab meeting cancelled <br> | |||
* Email me times that you CANNOT make for lab meetings starting May 13th. <br> | |||
'''‡ Flu R01:Integration''' <br> | |||
* meetings every other M 1-3pm. 725. <br> | |||
** SEQUENCING WORK<br> | |||
* for PCR just do more reactions, pool and etoh precipitate. <br> | |||
** For HDA, we need to explore cloning. Waiting for the competent cells. <br> | |||
** dxs PCR primers worked for HDA in tube. Still no positive results on chip. <br> | |||
* Check other methods of heating. <br> | |||
† HDA (Lead:Sonali)<br> | |||
* IBC Approval or 720 to work with real C.diff samples. <br> | |||
* Patient Samples - dilution experiments (MM/SH) <br> | |||
* Start planning R01 for Submission on '''10/5/10.''' MM, CMK. <br> | |||
* HDA mix in dried form - Expecting it from BH -- ON HOLD. <br> | |||
* <br> | |||
* QQ preliminary chip work <br> | |||
* Sequence all output <br> | |||
* 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br> | |||
<br> | |||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | |||
* Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time <br> | |||
* Straight 1mm and 4mm PDMS-Done <br> | |||
* Try collection vs dilutions, collection vs time, need binding constant to quantify results. <br> | |||
* <br> | |||
<br> | |||
† SPE Column Optimization for DNA/RNA. (Lead: ) <br> | |||
* (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | |||
* <br> | |||
<br> | |||
† Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing) <br> | |||
* Real copy number of previous human samples. So QQ is working with the higher concentration samples. Range is 10^6-10^8 of copy number/ml. <br> | |||
* REVISIT the % recovery number. <br> | |||
* QQ tried: more enzyme, Tween 20, BSA amounts. <br> | |||
* integrated flu chip paper has been send to cathie, wait for revision. | |||
<br> | |||
* PCR1 will combine with PCR2, waitting for revision (CMK)<br> | |||
<br> | |||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | |||
* Discuss Project Goals Today <br> | |||
<br> | |||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | |||
* New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED NEXT WEEK (4/20). <br> | |||
<br> | |||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | |||
* Paper on HotDog - with CMK/ASB <br> | |||
<br> | |||
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | |||
* Evap paper - Back to Jane 4/29. <br> | |||
<br> | |||
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | |||
'''* Cathie sent latest draft to MCK.<br>''' | |||
<br> | |||
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | |||
* Sample collection ongoing. <br> | |||
<br> | |||
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | |||
*.<br> | |||
* Frank: Four-week storage results sent to PATH group. +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng). Longer-term (2, 3, 4-month) in storage. Currently writing up final report for senior project. After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.<br> | |||
* Sean: Data for 700 nm and 150 nm analyzed and it shows that 700 is better overall, and is best in the 60 - 80 SPE range. Final report almost complete and due tomorrow (4/23). I will get stuff together and leave my notebook for future research as well. <br> | |||
* Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage. Final Report draft submitted, due tomorrow. Presentation next Friday 4/30. Lab notebook will be updated and ready for future PATH SNAP workers.<br> | |||
<br> | |||
'''‡ IIH Senior Project.'''<br> | |||
* Ordered new HRP substrate. waiting for delivery | |||
* Will run ELISA in chip and image for standard curve. | |||
* finalizing report | |||
Revision as of 08:19, 29 April 2010
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29 April 2010 Lab Meeting
‡ Announcements
‡ Flu R01:Integration
† HDA (Lead:Sonali)
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