Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Cmklapperich (talk | contribs) No edit summary |
|||
| (5 intermediate revisions by 4 users not shown) | |||
| Line 8: | Line 8: | ||
=22 April 2010 Lab Meeting= | =22 April 2010 Lab Meeting= | ||
'''‡ Announcements'''<br> | '''‡ Announcements'''<br> | ||
* May 6 Lab meeting cancelled <br> | |||
* Send me any conflicts for summer lab meeting time. <br> | |||
* Sr. Project Presentation practice at 4/29 meeting. <br> | |||
'''‡ Flu R01:Integration''' <br> | '''‡ Flu R01:Integration''' <br> | ||
* meetings every other M 1-3pm. 705. <br> | * meetings every other M 1-3pm. 705. <br> | ||
* QQ gone at Oakridge conf. <br> | |||
** SEQUENCING WORK<br> | ** SEQUENCING WORK<br> | ||
* for PCR just do more reactions, pool and etoh precipitate. <br> | * for PCR just do more reactions, pool and etoh precipitate. <br> | ||
** For HDA, we need to explore cloning. <br> | ** For HDA, we need to explore cloning. Waiting for the competent cells. <br> | ||
** dxs PCR primers worked for HDA in tube. Still no positive results on chip. <br> | |||
* Check other methods of heating. <br> | |||
† HDA (Lead | † HDA (Lead:Sonali)<br> | ||
* IBC Approval or 720 to work with real C.diff samples. <br> | * IBC Approval or 720 to work with real C.diff samples. <br> | ||
* Patient Samples - dilution experiments (MM/SH) <br> | * Patient Samples - dilution experiments (MM/SH) <br> | ||
* Start planning R01 for Submission on '''10/5/10.''' MM, CMK. <br> | * Start planning R01 for Submission on '''10/5/10.''' MM, CMK. <br> | ||
* HDA mix in dried form - Expecting it from BH -- ON HOLD. <br> | * HDA mix in dried form - Expecting it from BH -- ON HOLD. <br> | ||
* | * <br> | ||
* QQ preliminary chip work <br> | * QQ preliminary chip work <br> | ||
* Sequence all output <br> | * Sequence all output <br> | ||
| Line 32: | Line 36: | ||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | † Sample Concentration (Lead: Jane, Team: Jaephil) <br> | ||
* Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time <br> | * Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time <br> | ||
* Straight 1mm and 4mm PDMS- | * Straight 1mm and 4mm PDMS-Done <br> | ||
* Try collection vs dilutions, collection vs time, need binding constant to quantify results. <br> | * Try collection vs dilutions, collection vs time, need binding constant to quantify results. <br> | ||
* | * <br> | ||
<br> | <br> | ||
† SPE Column Optimization for DNA/RNA. (Lead: | † SPE Column Optimization for DNA/RNA. (Lead: ) <br> | ||
* (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | * (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | ||
| Line 72: | Line 74: | ||
*.<br> | *.<br> | ||
* Frank: Four-week storage results sent to PATH group. +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng). Longer-term (2, 3, 4-month) in storage. Currently writing up final report for senior project. After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.<br> | * Frank: Four-week storage results sent to PATH group. +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng). Longer-term (2, 3, 4-month) in storage. Currently writing up final report for senior project. After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.<br> | ||
* Sean: | * Sean: Data for 700 nm and 150 nm analyzed and it shows that 700 is better overall, and is best in the 60 - 80 SPE range. Final report almost complete and due tomorrow (4/23). I will get stuff together and leave my notebook for future research as well. <br> | ||
* Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage.<br> | * Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage. Final Report draft submitted, due tomorrow. Presentation next Friday 4/30. Lab notebook will be updated and ready for future PATH SNAP workers.<br> | ||
<br> | <br> | ||
'''‡ IIH Senior Project.'''<br> | '''‡ IIH Senior Project.'''<br> | ||
* ELISA | * Ordered new HRP substrate. waiting for delivery | ||
* | * Will run ELISA in chip and image for standard curve. | ||
* finalizing report | |||
Revision as of 12:04, 22 April 2010
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
22 April 2010 Lab Meeting‡ Announcements
‡ Flu R01:Integration
† HDA (Lead:Sonali)
| |
