Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/22: Difference between revisions
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=22 April 2010 Lab Meeting= | |||
'''‡ Announcements'''<br> | |||
'''‡ Flu R01:Integration''' <br> | |||
* meetings every other M 1-3pm. 705. <br> | |||
** SEQUENCING WORK<br> | |||
* for PCR just do more reactions, pool and etoh precipitate. <br> | |||
** For HDA, we need to explore cloning. <br> | |||
† HDA (Lead: Jaephil, Team:Sonali)<br> | |||
* IBC Approval or 720 to work with real C.diff samples. <br> | |||
* Patient Samples - dilution experiments (MM/SH) <br> | |||
* Start planning R01 for Submission on '''10/5/10.''' MM, CMK. <br> | |||
* HDA mix in dried form - Expecting it from BH -- ON HOLD. <br> | |||
* warm start here (Give some to Lisa)<br> | |||
* QQ preliminary chip work <br> | |||
* Sequence all output <br> | |||
* 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br> | |||
<br> | |||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | |||
* Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time <br> | |||
* Straight 1mm and 4mm PDMS-Teflon channel adhesion test pending PCR result. <br> | |||
* Try collection vs dilutions, collection vs time, need binding constant to quantify results. <br> | |||
* Samples expected from Fauchet group. Contact today. ??? - CMK will check again. <br> | |||
<br> | |||
† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | |||
<br> | |||
* http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5155018 <br> | |||
* (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | |||
* <br> | |||
<br> | |||
† Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing) <br> | |||
* Real copy number of previous human samples. So QQ is working with the higher concentration samples. Range is 10^6-10^8 of copy number/ml. <br> | |||
* REVISIT the % recovery number. <br> | |||
* QQ tried: more enzyme, Tween 20, BSA amounts. <br> | |||
* integrated flu chip paper has been send to cathie, wait for revision. | |||
<br> | |||
* PCR1 will combine with PCR2, waitting for revision (CMK)<br> | |||
<br> | |||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | |||
* See email notes. <br> | |||
<br> | |||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | |||
* New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED NEXT WEEK (4/20). <br> | |||
<br> | |||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | |||
* Paper on HotDog - with CMK/ASB <br> | |||
<br> | |||
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | |||
* Evap paper - Additional experiment for Fig 3. JD says will send by Friday (4/16). <br> | |||
<br> | |||
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | |||
'''* Cathie will submit paper.<br>''' | |||
<br> | |||
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | |||
* Sample collection ongoing. <br> | |||
<br> | |||
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | |||
*.<br> | |||
* Frank: Two- and three-week storage experiments completed. Four-week to be done next Wed. Longer-term (2, 3, 4-month) in storage. +/- carrier RNA not yet completed. Currently writing up final report.<br> | |||
* Sean: First set of data is in (for the 700 nm silica straws). Today (4/15) I will finish the straws for the 150 nm silica and the SPE batters for the 14 nm silica. Tomorrow I plan on running and PCR'ing samples for the 150 nm silica straws as well as making the straws for the 14 nm silica. On Saturday I will run and PCR samples for the last set of straws. <br> | |||
* Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage.<br> | |||
<br> | |||
'''‡ IIH Senior Project.'''<br> | |||
* ELISA done, Talking to Phil Allen about proper imaging | |||
* Writing report | |||
Revision as of 07:34, 22 April 2010
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22 April 2010 Lab Meeting‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
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